PSY3/YLR376C Summary Help

Standard Name PSY3 1
Systematic Name YLR376C
Feature Type ORF, Verified
Description Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function; structural analysis reveals a similar DNA-binding region in both Psy3p and Csm2p and that both regions work together to form a single DNA binding site; deletion of PSY3 results in a mutator phenotype; deletion increases sensitivity to anticancer drugs oxaliplatin and cisplatin but not mitomycin C (1, 2, 3, 4, 5, 6)
Name Description Platinum SensitivitY 1
Chromosomal Location
ChrXII:873554 to 872826 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All PSY3 GO evidence and references
  View Computational GO annotations for PSY3
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 79 genes
Resources
Classical genetics
null
Large-scale survey
null
Resources
74 total interaction(s) for 46 unique genes/features.
Physical Interactions
  • Affinity Capture-RNA: 2
  • Co-crystal Structure: 2
  • Co-purification: 1
  • Reconstituted Complex: 1
  • Two-hybrid: 9

Genetic Interactions
  • Negative Genetic: 31
  • Phenotypic Enhancement: 5
  • Phenotypic Suppression: 1
  • Positive Genetic: 10
  • Synthetic Growth Defect: 4
  • Synthetic Lethality: 1
  • Synthetic Rescue: 7

Resources
Expression Summary
histogram
Resources
Length (a.a.) 242
Molecular Weight (Da) 28,251
Isoelectric Point (pI) 8.17
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXII:873554 to 872826 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..729 873554..872826 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004368
References cited on this page View Complete Literature Guide for PSY3
1) Wu HI, et al.  (2004) Genome-wide identification of genes conferring resistance to the anticancer agents cisplatin, oxaliplatin, and mitomycin C. Cancer Res 64(11):3940-8
2) Huang ME, et al.  (2003) A genomewide screen in Saccharomyces cerevisiae for genes that suppress the accumulation of mutations. Proc Natl Acad Sci U S A 100(20):11529-34
3) Mankouri HW, et al.  (2007) Shu proteins promote the formation of homologous recombination intermediates that are processed by sgs1-rmi1-top3. Mol Biol Cell 18(10):4062-73
4) Ball LG, et al.  (2009) The yeast Shu complex couples error-free post-replication repair to homologous recombination. Mol Microbiol 73(1):89-102
5) Bernstein KA, et al.  (2011) The Shu complex, which contains Rad51 paralogues, promotes DNA repair through inhibition of the Srs2 anti-recombinase. Mol Biol Cell 22(9):1599-607
6) Tao Y, et al.  (2012) Structural analysis of Shu proteins reveals a DNA binding role essential for resisting damage. J Biol Chem 287(24):20231-9