PST2/YDR032C Summary Help

Standard Name PST2 1
Systematic Name YDR032C
Feature Type ORF, Verified
Description Protein with similarity to a family of flavodoxin-like proteins; induced by oxidative stress in a Yap1p dependent manner; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress; PST2 has a paralog, RFS1, that arose from the whole genome duplication (2, 3, 4, 5, 6, 7, 8)
Name Description Protoplasts-SecreTed 9
Chromosomal Location
ChrIV:504695 to 504099 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All PST2 GO evidence and references
  View Computational GO annotations for PST2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 6 genes
Resources
Classical genetics
null
Large-scale survey
null
Resources
101 total interaction(s) for 79 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 23
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 1
  • Biochemical Activity: 1
  • Co-purification: 1
  • PCA: 32
  • Two-hybrid: 9

Genetic Interactions
  • Negative Genetic: 27
  • Positive Genetic: 2
  • Synthetic Haploinsufficiency: 1
  • Synthetic Rescue: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 198
Molecular Weight (Da) 20,966
Isoelectric Point (pI) 5.61
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:504695 to 504099 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..597 504695..504099 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002439
References cited on this page View Complete Literature Guide for PST2
1) Aittamaa M, et al.  (2001) Functional analysis of six ORFs from Saccharomyces cerevisiae chromosome IV: two-spored asci produced by disruptant of YDR027c and strain-dependent DNA heterogeneity around YDR036c. Yeast 18(10):931-41
2) Lee J, et al.  (1999) Yap1 and Skn7 control two specialized oxidative stress response regulons in yeast. J Biol Chem 274(23):16040-6
3) Grandori R and Carey J  (1994) Six new candidate members of the alpha/beta twisted open-sheet family detected by sequence similarity to flavodoxin. Protein Sci 3(12):2185-93
4) Huh WK, et al.  (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91
5) Sickmann A, et al.  (2003) The proteome of Saccharomyces cerevisiae mitochondria. Proc Natl Acad Sci U S A 100(23):13207-12
6) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
7) Reinders J, et al.  (2006) Toward the complete yeast mitochondrial proteome: multidimensional separation techniques for mitochondrial proteomics. J Proteome Res 5(7):1543-54
8) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
9) Pardo M, et al.  (2000) A proteomic approach for the study of Saccharomyces cerevisiae cell wall biogenesis. Electrophoresis 21(16):3396-410