PSF3/YOL146W Summary Help

PSF3 BASIC INFORMATION

Standard Name PSF3
Systematic Name YOL146W
Feature Type ORF, Verified
Description Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery (1, 2 and see Summary Paragraph)
Name Description Partner of Sld Five 1
GO Annotations All PSF3 GO evidence and references
    View Computational GO annotations for PSF3
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Mutant Phenotype All PSF3 Phenotype details and references
Classical genetics
conditional
Large-scale survey
conditional
null
reduction of function
Interactions PSF3 All interactions details and references
  View additional details at BioGRID
24 total interaction(s) for 12 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 11
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 2
  • Co-purification: 1
  • Reconstituted Complex: 5
Genetic Interactions
  • Dosage Rescue: 4
Sequence Information
ChrXV:48863 to 49447 | ORF Map | GBrowse
Gbrowse
Last Update Coordinates: 2006-01-05 | Sequence: 2003-09-22
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..585 48863..49447 2006-01-05 2003-09-22
Post-translational Modifications PhosphoGRID | PhosphoPep Database
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | UniProtKB
Primary SGDIDS000005506

PSF3 RESOURCES

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SGD ORF map
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Expression Summary histogram

ADDITIONAL INFORMATION for PSF3

SUMMARY PARAGRAPH for PSF3

The GINS complex (named for Go, Ichi, Nii, and San for five, one, two, and three in Japanese) is composed of four subunits, encoded by SLD5, PSF1, PSF2, and PSF3. In S. cerevisiae, it was first identified by genetic and biochemical methods to determine factors interacting with Sld5p. Genetic interactions between these four genes also suggest that they act together (1). The GINS complex was independently isolated in a large scale screen for cell cycle defects (2). A similar complex is found in Xenopus and has a ring-like structure (3). In yeast, all four genes are essential and cells defective in SLD5, PSF1, or PSF2 are impaired in their ability to replicate DNA (1, 2). The complex localizes to origins of DNA replication (1, 2) and Sld5p was previously implicated as functioning in DNA replication due to its genetic interaction with DPB11 (4). Additional genetic and biochemical interactions of the GINS complex with Dpb11p, Dpb2p, and Sld3p suggest that it functions in some way at the replication fork during DNA synthesis (1).

Last updated: 2005-12-02

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for PSF3]

1) Takayama Y, et al.  (2003) GINS, a novel multiprotein complex required for chromosomal DNA replication in budding yeast. Genes Dev 17(9):1153-65
2) Kanemaki M, et al.  (2003) Functional proteomic identification of DNA replication proteins by induced proteolysis in vivo. Nature 423(6941):720-4
3) Kubota Y, et al.  (2003) A novel ring-like complex of Xenopus proteins essential for the initiation of DNA replication. Genes Dev 17(9):1141-52
4) Kamimura Y, et al.  (1998) Sld2, which interacts with Dpb11 in Saccharomyces cerevisiae, is required for chromosomal DNA replication. Mol Cell Biol 18(10):6102-9