PSF2/YJL072C Summary Help

Standard Name PSF2 1
Systematic Name YJL072C
Alias CDC102
Feature Type ORF, Verified
Description Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p); complex is localized to DNA replication origins and implicated in assembly of the DNA replication machinery (1, 2 and see Summary Paragraph)
Name Description Partner of Sld Five 1
Chromosomal Location
ChrX:305862 to 305221 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All PSF2 GO evidence and references
  View Computational GO annotations for PSF2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 2 genes
Classical genetics
Large-scale survey
reduction of function
64 total interaction(s) for 22 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 14
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 22
  • Co-purification: 1
  • Reconstituted Complex: 17
  • Two-hybrid: 4

Genetic Interactions
  • Dosage Rescue: 1
  • Synthetic Growth Defect: 1
  • Synthetic Lethality: 3

Expression Summary
Length (a.a.) 213
Molecular Weight (Da) 25,061
Isoelectric Point (pI) 6.64
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrX:305862 to 305221 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..642 305862..305221 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003608

The GINS complex (named for Go, Ichi, Nii, and San for five, one, two, and three in Japanese) is composed of four subunits, encoded by SLD5, PSF1, PSF2, and PSF3. In S. cerevisiae, it was first identified by genetic and biochemical methods to determine factors interacting with Sld5p. Genetic interactions between these four genes also suggest that they act together (1). The GINS complex was independently isolated in a large scale screen for cell cycle defects (2). A similar complex is found in Xenopus and has a ring-like structure (3). In yeast, all four genes are essential and cells defective in SLD5, PSF1, or PSF2 are impaired in their ability to replicate DNA (1, 2). The complex localizes to origins of DNA replication (1, 2) and Sld5p was previously implicated as functioning in DNA replication due to its genetic interaction with DPB11 (4). Additional genetic and biochemical interactions of the GINS complex with Dpb11p, Dpb2p, and Sld3p suggest that it functions in some way at the replication fork during DNA synthesis (1).

Last updated: 2005-12-02 Contact SGD

References cited on this page View Complete Literature Guide for PSF2
1) Takayama Y, et al.  (2003) GINS, a novel multiprotein complex required for chromosomal DNA replication in budding yeast. Genes Dev 17(9):1153-65
2) Kanemaki M, et al.  (2003) Functional proteomic identification of DNA replication proteins by induced proteolysis in vivo. Nature 423(6941):720-4
3) Kubota Y, et al.  (2003) A novel ring-like complex of Xenopus proteins essential for the initiation of DNA replication. Genes Dev 17(9):1141-52
4) Kamimura Y, et al.  (1998) Sld2, which interacts with Dpb11 in Saccharomyces cerevisiae, is required for chromosomal DNA replication. Mol Cell Biol 18(10):6102-9