PRS5/YOL061W Summary Help

Standard Name PRS5 1
Systematic Name YOL061W
Feature Type ORF, Verified
Description 5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase; synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; forms cytoplasmic foci upon DNA replication stress (1, 2, 3, 4)
Name Description PhosphoRibosylpyrophosphate Synthetase 5
Chromosomal Location
ChrXV:212244 to 213734 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All PRS5 GO evidence and references
  View Computational GO annotations for PRS5
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
High-throughput
Regulators 6 genes
Resources
Pathways
Classical genetics
null
Large-scale survey
null
overexpression
repressible
Resources
75 total interaction(s) for 54 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 16
  • PCA: 5
  • Reconstituted Complex: 9
  • Two-hybrid: 9

Genetic Interactions
  • Negative Genetic: 22
  • Positive Genetic: 7
  • Synthetic Growth Defect: 2
  • Synthetic Lethality: 4
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 496
Molecular Weight (Da) 53,504
Isoelectric Point (pI) 7.26
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXV:212244 to 213734 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2006-01-05 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1491 212244..213734 2006-01-05 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005422
References cited on this page View Complete Literature Guide for PRS5
1) Hernando Y, et al.  (1998) PRS5, the fifth member of the phosphoribosyl pyrophosphate synthetase gene family in Saccharomyces cerevisiae, is essential for cell viability in the absence of either PRS1 or PRS3. J Bacteriol 180(23):6404-7
2) Hernando Y, et al.  (1999) Genetic analysis and enzyme activity suggest the existence of more than one minimal functional unit capable of synthesizing phosphoribosyl pyrophosphate in Saccharomyces cerevisiae. J Biol Chem 274(18):12480-7
3) Carter AT, et al.  (1997) PRS1 is a key member of the gene family encoding phosphoribosylpyrophosphate synthetase in Saccharomyces cerevisiae. Mol Gen Genet 254(2):148-56
4) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
5) Carter AT, et al.  (1994) Phosphoribosylpyrophosphate synthetase (PRS): a new gene family in Saccharomyces cerevisiae. Yeast 10(8):1031-44