PRS1/YKL181W Summary Help

Standard Name PRS1 1
Systematic Name YKL181W
Alias PRP1
Feature Type ORF, Verified
Description 5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase; synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; plays a key role in cell wall integrity (CWI) pathway; one of five related enzymes, which are active as heteromultimeric complexes; missense mutations in human homolog PRPS1 are associated with neuropathic Arts syndrome and Charcot-Marie Tooth (CMTX5) disease (1, 2, 3, 4)
Name Description PhosphoRibosylpyrophosphate Synthetase 5
Chromosomal Location
ChrXI:107316 to 108599 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All PRS1 GO evidence and references
  View Computational GO annotations for PRS1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 10 genes
Resources
Pathways
Classical genetics
null
Large-scale survey
null
Resources
53 total interaction(s) for 37 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 14
  • Affinity Capture-RNA: 2
  • Biochemical Activity: 6
  • Co-purification: 1
  • PCA: 7
  • Reconstituted Complex: 11
  • Two-hybrid: 7

Genetic Interactions
  • Synthetic Growth Defect: 1
  • Synthetic Lethality: 4

Resources
Expression Summary
histogram
Resources
Length (a.a.) 427
Molecular Weight (Da) 47,047
Isoelectric Point (pI) 5.53
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXI:107316 to 108599 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1284 107316..108599 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001664
References cited on this page View Complete Literature Guide for PRS1
1) Carter AT, et al.  (1997) PRS1 is a key member of the gene family encoding phosphoribosylpyrophosphate synthetase in Saccharomyces cerevisiae. Mol Gen Genet 254(2):148-56
2) Hernando Y, et al.  (1999) Genetic analysis and enzyme activity suggest the existence of more than one minimal functional unit capable of synthesizing phosphoribosyl pyrophosphate in Saccharomyces cerevisiae. J Biol Chem 274(18):12480-7
3) Hernando Y, et al.  (1998) PRS5, the fifth member of the phosphoribosyl pyrophosphate synthetase gene family in Saccharomyces cerevisiae, is essential for cell viability in the absence of either PRS1 or PRS3. J Bacteriol 180(23):6404-7
4) Ugbogu EA, et al.  (2013) The contribution of the nonhomologous region of Prs1 to the maintenance of cell wall integrity and cell viability. FEMS Yeast Res 13(3):291-301
5) Carter AT, et al.  (1994) Phosphoribosylpyrophosphate synthetase (PRS): a new gene family in Saccharomyces cerevisiae. Yeast 10(8):1031-44