PRP8/YHR165C Summary Help

PRP8 BASIC INFORMATION

Standard Name PRP8
Systematic Name YHR165C
Alias DBF3 , DNA39 1 , RNA8 , SLT21 2 , USA2 3
Feature Type ORF, Verified
Description Component of the U4/U6-U5 snRNP complex, involved in the second catalytic step of splicing; mutations of human Prp8 cause retinitis pigmentosa (4, 5, 6)
Name Description Pre-mRNA Processing 7
GO Annotations All PRP8 GO evidence and references
    View Computational GO annotations for PRP8
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Mutant Phenotype All PRP8 Phenotype details and references
Classical genetics
conditional
null
Interactions PRP8 All interactions details and references
277 total interaction(s) for 98 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 197
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 16
  • Co-fractionation: 4
  • Co-localization: 2
  • Co-purification: 2
  • Protein-peptide: 2
  • Reconstituted Complex: 2
  • Two-hybrid: 25
Genetic Interactions
  • Dosage Lethality: 2
  • Dosage Rescue: 2
  • Phenotypic Suppression: 1
  • Synthetic Growth Defect: 1
  • Synthetic Lethality: 11
  • Synthetic Rescue: 9
Sequence Information
ChrVIII:436950 to 429709 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Last Update Coordinates: 2005-11-07 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..7242 436950..429709 2005-11-07 1996-07-31
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | UniProtKB
Primary SGDIDS000001208

PRP8 RESOURCES

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SGD ORF mapGBrowse
SGD ORF map
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  • Functional Analysis

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Expression Summary histogram

ADDITIONAL INFORMATION for PRP8

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for PRP8]

1) Dumas LB, et al.  (1982) New temperature-sensitive mutants of Saccharomyces cerevisiae affecting DNA replication. Mol Gen Genet 187(1):42-6
2) Xu D, et al.  (1998) Synthetic lethality of yeast slt mutations with U2 small nuclear RNA mutations suggests functional interactions between U2 and U5 snRNPs that are important for both steps of pre-mRNA splicing. Mol Cell Biol 18(4):2055-66
3) Awasthi S, et al.  (2001) New roles for the Snp1 and Exo84 proteins in yeast pre-mRNA splicing. J Biol Chem 276(33):31004-15
4) Stevens SW and Abelson J  (1999) Purification of the yeast U4/U6.U5 small nuclear ribonucleoprotein particle and identification of its proteins. Proc Natl Acad Sci U S A 96(13):7226-31
5) Umen JG and Guthrie C  (1995) The second catalytic step of pre-mRNA splicing. RNA 1(9):869-85
6) Boon KL, et al.  (2007) prp8 mutations that cause human retinitis pigmentosa lead to a U5 snRNP maturation defect in yeast. Nat Struct Mol Biol 14(11):1077-83
7) Vijayraghavan U, et al.  (1989) Isolation and characterization of pre-mRNA splicing mutants of Saccharomyces cerevisiae. Genes Dev 3(8):1206-16