PRP8/YHR165C Summary Help

Standard Name PRP8 1
Systematic Name YHR165C
Alias DBF3 2 , DNA39 3 , RNA8 4 , SLT21 5 , USA2 6
Feature Type ORF, Verified
Description Component of U4/U6-U5 snRNP complex; involved in second catalytic step of splicing; participates in spliceosomal assembly through its interaction with U1 snRNA; largest and most evolutionarily conserved protein of the spliceosome; mutations in its human ortholog, PRPF8, cause Retinitis pigmentosa and missplicing in Myelodysplastic syndrome. (7, 8, 9, 10, 11)
Name Description Pre-mRNA Processing 1
Chromosomal Location
ChrVIII:436948 to 429707 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All PRP8 GO evidence and references
  View Computational GO annotations for PRP8
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 3 genes
Classical genetics
321 total interaction(s) for 117 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 217
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 21
  • Co-crystal Structure: 3
  • Co-fractionation: 6
  • Co-localization: 2
  • Co-purification: 2
  • Protein-peptide: 2
  • Reconstituted Complex: 4
  • Two-hybrid: 25

Genetic Interactions
  • Dosage Lethality: 2
  • Dosage Rescue: 2
  • Negative Genetic: 7
  • Phenotypic Enhancement: 1
  • Synthetic Growth Defect: 5
  • Synthetic Lethality: 11
  • Synthetic Rescue: 9

Expression Summary
Length (a.a.) 2,413
Molecular Weight (Da) 279,501
Isoelectric Point (pI) 7.31
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrVIII:436948 to 429707 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..7242 436948..429707 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001208
References cited on this page View Complete Literature Guide for PRP8
1) Vijayraghavan U, et al.  (1989) Isolation and characterization of pre-mRNA splicing mutants of Saccharomyces cerevisiae. Genes Dev 3(8):1206-16
2) Johnston LH and Thomas AP  (1982) The isolation of new DNA synthesis mutants in the yeast Saccharomyces cerevisiae. Mol Gen Genet 186(3):439-44
3) Dumas LB, et al.  (1982) New temperature-sensitive mutants of Saccharomyces cerevisiae affecting DNA replication. Mol Gen Genet 187(1):42-6
4) Hartwell LH, et al.  (1970) Identification of ten genes that control ribosome formation in yeast. Mol Gen Genet 109(1):42-56
5) Xu D, et al.  (1998) Synthetic lethality of yeast slt mutations with U2 small nuclear RNA mutations suggests functional interactions between U2 and U5 snRNPs that are important for both steps of pre-mRNA splicing. Mol Cell Biol 18(4):2055-66
6) Awasthi S, et al.  (2001) New roles for the Snp1 and Exo84 proteins in yeast pre-mRNA splicing. J Biol Chem 276(33):31004-15
7) Stevens SW and Abelson J  (1999) Purification of the yeast U4/U6.U5 small nuclear ribonucleoprotein particle and identification of its proteins. Proc Natl Acad Sci U S A 96(13):7226-31
8) Umen JG and Guthrie C  (1995) The second catalytic step of pre-mRNA splicing. RNA 1(9):869-85
9) Boon KL, et al.  (2007) prp8 mutations that cause human retinitis pigmentosa lead to a U5 snRNP maturation defect in yeast. Nat Struct Mol Biol 14(11):1077-83
10) Li X, et al.  (2013) Comprehensive in vivo RNA-binding site analyses reveal a role of Prp8 in spliceosomal assembly. Nucleic Acids Res 41(6):3805-18
11) Kurtovic-Kozaric A, et al.  (2014) PRPF8 defects cause missplicing in myeloid malignancies. Leukemia ()