PRP8/YHR165C Summary Help

Standard Name PRP8 1
Systematic Name YHR165C
Alias DBF3 2 , DNA39 3 , RNA8 4 , SLT21 5 , USA2 6
Feature Type ORF, Verified
Description Component of U4/U6-U5 snRNP complex; involved in second catalytic step of splicing; participates in spliceosomal assembly through its interaction with U1 snRNA; largest and most evolutionarily conserved protein of the spliceosome; mutations in its human ortholog, PRPF8, cause Retinitis pigmentosa and missplicing in Myelodysplastic syndrome. (7, 8, 9, 10, 11)
Name Description Pre-mRNA Processing 1
Chromosomal Location
ChrVIII:436948 to 429707 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All PRP8 GO evidence and references
  View Computational GO annotations for PRP8
Molecular Function
Manually curated
High-throughput
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 3 genes
Resources
Classical genetics
conditional
null
Resources
318 total interaction(s) for 117 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 217
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 21
  • Co-crystal Structure: 2
  • Co-fractionation: 6
  • Co-localization: 2
  • Co-purification: 2
  • Protein-peptide: 2
  • Reconstituted Complex: 2
  • Two-hybrid: 25

Genetic Interactions
  • Dosage Lethality: 2
  • Dosage Rescue: 2
  • Negative Genetic: 7
  • Phenotypic Enhancement: 1
  • Synthetic Growth Defect: 5
  • Synthetic Lethality: 11
  • Synthetic Rescue: 9

Resources
Expression Summary
histogram
Resources
Length (a.a.) 2,413
Molecular Weight (Da) 279,501
Isoelectric Point (pI) 7.31
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVIII:436948 to 429707 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..7242 436948..429707 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001208
References cited on this page View Complete Literature Guide for PRP8
1) Vijayraghavan U, et al.  (1989) Isolation and characterization of pre-mRNA splicing mutants of Saccharomyces cerevisiae. Genes Dev 3(8):1206-16
2) Johnston LH and Thomas AP  (1982) The isolation of new DNA synthesis mutants in the yeast Saccharomyces cerevisiae. Mol Gen Genet 186(3):439-44
3) Dumas LB, et al.  (1982) New temperature-sensitive mutants of Saccharomyces cerevisiae affecting DNA replication. Mol Gen Genet 187(1):42-6
4) Hartwell LH, et al.  (1970) Identification of ten genes that control ribosome formation in yeast. Mol Gen Genet 109(1):42-56
5) Xu D, et al.  (1998) Synthetic lethality of yeast slt mutations with U2 small nuclear RNA mutations suggests functional interactions between U2 and U5 snRNPs that are important for both steps of pre-mRNA splicing. Mol Cell Biol 18(4):2055-66
6) Awasthi S, et al.  (2001) New roles for the Snp1 and Exo84 proteins in yeast pre-mRNA splicing. J Biol Chem 276(33):31004-15
7) Stevens SW and Abelson J  (1999) Purification of the yeast U4/U6.U5 small nuclear ribonucleoprotein particle and identification of its proteins. Proc Natl Acad Sci U S A 96(13):7226-31
8) Umen JG and Guthrie C  (1995) The second catalytic step of pre-mRNA splicing. RNA 1(9):869-85
9) Boon KL, et al.  (2007) prp8 mutations that cause human retinitis pigmentosa lead to a U5 snRNP maturation defect in yeast. Nat Struct Mol Biol 14(11):1077-83
10) Li X, et al.  (2013) Comprehensive in vivo RNA-binding site analyses reveal a role of Prp8 in spliceosomal assembly. Nucleic Acids Res 41(6):3805-18
11) Kurtovic-Kozaric A, et al.  (2014) PRPF8 defects cause missplicing in myeloid malignancies. Leukemia ()