PRP5/YBR237W Summary

PRP5/YBR237W Summary

Standard Name	PRP5
Systematic Name	YBR237W
Alias	RNA5
Feature Type	ORF, Verified
Description	RNA helicase in the DEAD-box family, necessary for prespliceosome formation, bridges U1 and U2 snRNPs and enables stable U2 snRNP association with intron RNA (1, 2, 3)
Name Description	Pre-mRNA Processing 4

Chromosomal Location	ChrII:691969 to 694518 \| ORF Map \| GBrowse

	Genetic position: 138 cM

Gene Ontology Annotations All PRP5 GO evidence and references

	View Computational GO annotations for PRP5
Molecular Function
Manually curated	RNA-dependent ATPase activity (IDA)
Biological Process
Manually curated	mRNA branch site recognition (TAS)
Cellular Component
High-throughput	nucleus (IDA)

Mutant phenotypes All PRP5 Phenotype evidence and references

Classical genetics
conditional	heat sensitivity: increased
Large-scale survey
conditional	resistance to benomyl: decreased
null	inviable
overexpression	vegetative growth: decreased
reduction of function	competitive fitness: increased
Resources	PROPHECY \| PhenoM \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All PRP5 Interaction evidence and references

	20 total interaction(s) for 16 unique genes/features.
Physical Interactions	Affinity Capture-MS: 2 Affinity Capture-RNA: 2 Biochemical Activity: 1 PCA: 1 Protein-RNA: 1 Two-hybrid: 2
Genetic Interactions	Dosage Rescue: 1 Negative Genetic: 1 Phenotypic Suppression: 1 Positive Genetic: 1 Synthetic Growth Defect: 4 Synthetic Lethality: 1 Synthetic Rescue: 2
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All PRP5 Protein evidence and references

Localization	YeastRC \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrII:691969 to 694518 | |

SGD ORF map

: 138 cM

Last Update

Coordinates: 2011-02-03 | Sequence: 1997-01-28

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..2550	691969..694518	2011-02-03	1997-01-28

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| E.C. \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000000441

References cited on this page View Complete Literature Guide for PRP5

1)	de la Cruz J, et al. (1999) Unwinding RNA in Saccharomyces cerevisiae: DEAD-box proteins and related families. Trends Biochem Sci 24(5):192-8
2)	Abu Dayyeh BK, et al. (2002) Probing interactions between the U2 small nuclear ribonucleoprotein and the DEAD-box protein, Prp5. J Biol Chem 277(23):20221-33
3)	Xu YZ, et al. (2004) Prp5 bridges U1 and U2 snRNPs and enables stable U2 snRNP association with intron RNA. EMBO J 23(2):376-85
4)	Vijayraghavan U, et al. (1989) Isolation and characterization of pre-mRNA splicing mutants of Saccharomyces cerevisiae. Genes Dev 3(8):1206-16