PRP43/YGL120C Summary

PRP43 BASIC INFORMATION

Standard Name	PRP43 1
Systematic Name	YGL120C
Feature Type	ORF, Verified
Description	RNA helicase in the DEAH-box family, functions in both RNA polymerase I and polymerase II transcript metabolism, involved in release of the lariat-intron from the spliceosome (1, 2, 3, 4) Also known as: JA1
Name Description	Pre-mRNA Processing 1

GO Annotations	All PRP43 GO evidence and references
	View Computational GO annotations for PRP43
Molecular Function
Manually curated	ATP-dependent RNA helicase activity (IDA, IMP)
Biological Process
Manually curated	maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) (IMP) maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) (IMP) maturation of SSU-rRNA (IGI) maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) (IMP) ribosomal large subunit biogenesis (IMP) rRNA processing (IMP) spliceosome disassembly (IDA, IGI)
Cellular Component
Manually curated	90S preribosome (IDA) post-mRNA release spliceosomal complex (IMP)
High-throughput	mitochondrion (IDA)

Mutant Phenotype	All PRP43 Phenotype details and references
Classical genetics
reduction of function	pre-mRNA accumulation: increased 27S pre-rRNA accumulation: decreased 20S pre-rRNA accumulation: decreased cold sensitivity: increased heat sensitivity: increased
Large-scale survey
null	inviable

Interactions	PRP43 All interactions details and references
	248 total interaction(s) for 150 unique genes/features.
Physical Interactions	Affinity Capture-MS: 210 Affinity Capture-Western: 7 Biochemical Activity: 1 Co-fractionation: 3 PCA: 11 Reconstituted Complex: 2 Two-hybrid: 8
Genetic Interactions	Dosage Lethality: 2 Synthetic Growth Defect: 1 Synthetic Lethality: 2 Synthetic Rescue: 1

Sequence Information

ChrVII:283943 to 281640 | |

Note: this feature is encoded on the Crick strand.

Last Update

Coordinates: 2004-07-20 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..2304	283943..281640	2004-07-20	1996-07-31

External Links	All Associated Seq \| E.C. \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| UniProtKB

Primary SGDID	S000003088

PRP43 RESOURCES

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SGD ORF map	GBrowse

Click on histogram for expression summary
Expression Summary

ADDITIONAL INFORMATION for PRP43

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for PRP43]

1)	Arenas JE and Abelson JN (1997) Prp43: An RNA helicase-like factor involved in spliceosome disassembly. Proc Natl Acad Sci U S A 94(22):11798-802
2)	de la Cruz J, et al. (1999) Unwinding RNA in Saccharomyces cerevisiae: DEAD-box proteins and related families. Trends Biochem Sci 24(5):192-8
3)	Martin A, et al. (2002) Prp43 is an essential RNA-dependent ATPase required for release of lariat-intron from the spliceosome. J Biol Chem 277(20):17743-50
4)	Combs DJ, et al. (2006) Prp43p is a DEAH-box spliceosome disassembly factor essential for ribosome biogenesis. Mol Cell Biol 26(2):523-34

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