PRP43/YGL120C Summary Help

Standard Name PRP43 1
Systematic Name YGL120C
Feature Type ORF, Verified
Description RNA helicase in the DEAH-box family; functions in both RNA polymerase I and polymerase II transcript metabolism; catalyzes removal of U2, U5, and U6 snRNPs from the postsplicing lariat-intron ribonucleoprotein complex; required for efficient biogenesis of both small- and large-subunit rRNAs; acts with Sqs1p to promote 20S to 18S rRNA processing catalyzed by endonuclease Nob1p (1, 2, 3, 4, 5, 6, 7)
Also known as: JA1
Name Description Pre-mRNA Processing 1
Chromosomal Location
ChrVII:283937 to 281634 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All PRP43 GO evidence and references
  View Computational GO annotations for PRP43
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 5 genes
Classical genetics
dominant negative
reduction of function
Large-scale survey
reduction of function
285 total interaction(s) for 175 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 238
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 10
  • Biochemical Activity: 1
  • Co-fractionation: 3
  • PCA: 11
  • Reconstituted Complex: 2
  • Two-hybrid: 8

Genetic Interactions
  • Dosage Lethality: 2
  • Positive Genetic: 1
  • Synthetic Growth Defect: 1
  • Synthetic Lethality: 6
  • Synthetic Rescue: 1

Expression Summary
Length (a.a.) 767
Molecular Weight (Da) 87,561
Isoelectric Point (pI) 6.38
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrVII:283937 to 281634 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..2304 283937..281634 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003088
References cited on this page View Complete Literature Guide for PRP43
1) Arenas JE and Abelson JN  (1997) Prp43: An RNA helicase-like factor involved in spliceosome disassembly. Proc Natl Acad Sci U S A 94(22):11798-802
2) de la Cruz J, et al.  (1999) Unwinding RNA in Saccharomyces cerevisiae: DEAD-box proteins and related families. Trends Biochem Sci 24(5):192-8
3) Martin A, et al.  (2002) Prp43 is an essential RNA-dependent ATPase required for release of lariat-intron from the spliceosome. J Biol Chem 277(20):17743-50
4) Combs DJ, et al.  (2006) Prp43p is a DEAH-box spliceosome disassembly factor essential for ribosome biogenesis. Mol Cell Biol 26(2):523-34
5) Leeds NB, et al.  (2006) The splicing factor Prp43p, a DEAH box ATPase, functions in ribosome biogenesis. Mol Cell Biol 26(2):513-22
6) Tanaka N, et al.  (2007) Ntr1 activates the Prp43 helicase to trigger release of lariat-intron from the spliceosome. Genes Dev 21(18):2312-25
7) Pertschy B, et al.  (2009) RNA helicase Prp43 and its co-factor Pfa1 promote 20 to 18 S rRNA processing catalyzed by the endonuclease Nob1. J Biol Chem 284(50):35079-91