Standard Name	PRP28 1
Systematic Name	YDR243C
Feature Type	ORF, Verified
Description	RNA helicase in the DEAD-box family, involved in RNA isomerization at the 5' splice site (2, 3)
Name Description	Pre-mRNA Processing

Chromosomal Location	ChrIV:950284 to 948518 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.
	Genetic position: 144.27 cM

	View Computational GO annotations for PRP28
Molecular Function
Manually curated	ATP-dependent RNA helicase activity (TAS)
Biological Process
Manually curated	cis assembly of pre-catalytic spliceosome (TAS)
Cellular Component
Manually curated	spliceosomal complex (TAS) U5 snRNP (IDA)

Classical genetics
conditional	unspliced pre-mRNA accumulation: increased cold sensitivity: increased
null	inviable
Large-scale survey
null	haploproficient inviable
reduction of function	competitive fitness: increased
Resources	PROPHECY | PhenoM | SCMD | ScreenTroll | Yeast Fitness Database

	79 total interaction(s) for 77 unique genes/features.
Physical Interactions	Affinity Capture-MS: 8 Affinity Capture-RNA: 1 Protein-RNA: 1
Genetic Interactions	Dosage Growth Defect: 1 Negative Genetic: 55 Positive Genetic: 4 Synthetic Growth Defect: 1 Synthetic Lethality: 4 Synthetic Rescue: 4
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrIV:950284 to 948518 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.
	Genetic position: 144.27 cM
Last Update	Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..1767 950284..948518 2011-02-03 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI1631 S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FL100 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain Y10 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000002651