PRP28/YDR243C Summary Help

Standard Name PRP28 1
Systematic Name YDR243C
Feature Type ORF, Verified
Description RNA helicase in the DEAD-box family; involved in RNA isomerization at the 5' splice site and for exchange of U6 for U1 snRNA at the 5' splice site (2, 3, 4)
Name Description Pre-mRNA Processing
Chromosomal Location
ChrIV:950284 to 948518 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Genetic position: 144.27 cM
Gene Ontology Annotations All PRP28 GO evidence and references
  View Computational GO annotations for PRP28
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 4 genes
Resources
Classical genetics
conditional
null
Large-scale survey
null
reduction of function
Resources
79 total interaction(s) for 77 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 8
  • Affinity Capture-RNA: 2
  • Protein-RNA: 1

Genetic Interactions
  • Dosage Growth Defect: 1
  • Negative Genetic: 55
  • Positive Genetic: 3
  • Synthetic Growth Defect: 1
  • Synthetic Lethality: 4
  • Synthetic Rescue: 4

Resources
Expression Summary
histogram
Resources
Length (a.a.) 588
Molecular Weight (Da) 66,641
Isoelectric Point (pI) 9.36
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:950284 to 948518 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Genetic position: 144.27 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1767 950284..948518 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002651
References cited on this page View Complete Literature Guide for PRP28
1) Strauss EJ and Guthrie C  (1991) A cold-sensitive mRNA splicing mutant is a member of the RNA helicase gene family. Genes Dev 5(4):629-41
2) Staley JP and Guthrie C  (1999) An RNA switch at the 5' splice site requires ATP and the DEAD box protein Prp28p. Mol Cell 3(1):55-64
3) de la Cruz J, et al.  (1999) Unwinding RNA in Saccharomyces cerevisiae: DEAD-box proteins and related families. Trends Biochem Sci 24(5):192-8
4) Price AM, et al.  (2014) An unanticipated early function of DEAD-box ATPase Prp28 during commitment to splicing is modulated by U5 snRNP protein Prp8. RNA 20(1):46-60