PRP22/YER013W Summary

PRP22 BASIC INFORMATION

Standard Name	PRP22 1
Systematic Name	YER013W
Feature Type	ORF, Verified
Description	DEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase, associates with lariat intermediates before the second catalytic step of splicing; mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes (2, 3, 4, 5, 6)
Name Description	Pre-mRNA Processing 1

GO Annotations	All PRP22 GO evidence and references
	View Computational GO annotations for PRP22
Molecular Function
Manually curated	ATP-dependent RNA helicase activity (IDA) contributes_to second spliceosomal transesterification activity (IDA, IMP)
Biological Process
Manually curated	generation of catalytic spliceosome for second transesterification step (IDA) spliceosome disassembly (IMP)
Cellular Component
Manually curated	U2-type catalytic step 2 spliceosome (IMP) U2-type post-spliceosomal complex (IMP)

Mutant Phenotype	All PRP22 Phenotype details and references
Classical genetics
conditional	unspliced intron-containing RNA accumulation: abnormal heat sensitivity: increased
Large-scale survey
null	filamentous growth: decreased invasive growth: absent inviable

Interactions	PRP22 All interactions details and references
	45 total interaction(s) for 37 unique genes/features.
Physical Interactions	Affinity Capture-MS: 9 Affinity Capture-RNA: 1 Biochemical Activity: 1 Two-hybrid: 8
Genetic Interactions	Dosage Rescue: 1 Phenotypic Enhancement: 15 Synthetic Growth Defect: 3 Synthetic Lethality: 3 Synthetic Rescue: 4

Sequence Information

ChrV:178840 to 182277 | |

Last Update

Coordinates: 1996-07-31 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..3438	178840..182277	1996-07-31	1996-07-31

External Links	All Associated Seq \| E.C. \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| UniProtKB

Primary SGDID	S000000815

PRP22 RESOURCES

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SGD ORF map	GBrowse

Click on histogram for expression summary
Expression Summary

ADDITIONAL INFORMATION for PRP22

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for PRP22]

1)	Vijayraghavan U, et al. (1989) Isolation and characterization of pre-mRNA splicing mutants of Saccharomyces cerevisiae. Genes Dev 3(8):1206-16
2)	Schwer B and Gross CH (1998) Prp22, a DExH-box RNA helicase, plays two distinct roles in yeast pre-mRNA splicing. EMBO J 17(7):2086-94
3)	de la Cruz J, et al. (1999) Unwinding RNA in Saccharomyces cerevisiae: DEAD-box proteins and related families. Trends Biochem Sci 24(5):192-8
4)	Wagner JD, et al. (1998) The DEAH-box protein PRP22 is an ATPase that mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes. EMBO J 17(10):2926-37
5)	McPheeters DS and Muhlenkamp P (2003) Spatial organization of protein-RNA interactions in the branch site-3' splice site region during pre-mRNA splicing in yeast. Mol Cell Biol 23(12):4174-86
6)	Umen JG and Guthrie C (1995) The second catalytic step of pre-mRNA splicing. RNA 1(9):869-85

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