Standard Name	PRP2
Systematic Name	YNR011C
Alias	RNA2
Feature Type	ORF, Verified
Description	RNA-dependent ATPase in the DEAH-box family, required for activation of the spliceosome before the first transesterification step in RNA splicing; orthologous to human protein DHX16 (1, 2)
Name Description	Pre-mRNA Processing 3

Chromosomal Location	ChrXIV:646950 to 644320 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.
	Genetic position: 8 cM

	View Computational GO annotations for PRP2
Molecular Function
Manually curated	RNA-dependent ATPase activity (IDA)
Biological Process
Manually curated	generation of catalytic spliceosome for first transesterification step (IDA)
Cellular Component
Manually curated	colocalizes_with U2-type catalytic step 1 spliceosome (IDA, IMP)

Classical genetics
conditional	unspliced intron-containing RNA accumulation: increased heat sensitivity: increased sporulation: absent
overexpression	inviable
Large-scale survey
null	haploinsufficient inviable
reduction of function	competitive fitness: increased resistance to methyl methanesulfonate: decreased resistance to hydroxyurea: decreased
Resources	PROPHECY | PhenoM | SCMD | ScreenTroll | Yeast Fitness Database

	95 total interaction(s) for 84 unique genes/features.
Physical Interactions	Affinity Capture-MS: 53 Affinity Capture-Western: 3 Protein-peptide: 5 Two-hybrid: 5
Genetic Interactions	Dosage Rescue: 2 Negative Genetic: 1 Phenotypic Enhancement: 6 Positive Genetic: 14 Synthetic Growth Defect: 3 Synthetic Lethality: 2 Synthetic Rescue: 1
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | CGD Homologs | CandidaDB Homologs | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrXIV:646950 to 644320 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.
	Genetic position: 8 cM
Last Update	Coordinates: 2011-02-03 | Sequence: 1997-01-28
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..2631 646950..644320 2011-02-03 1997-01-28
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI1631 S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CEN.PK113-7D S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain PW5 S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain YJM789 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000005294