PRP2/YNR011C Summary Help

Standard Name PRP2 1
Systematic Name YNR011C
Alias RNA2 2
Feature Type ORF, Verified
Description RNA-dependent DExD/H-box ATPase; required for activation of spliceosome before first transesterification step in RNA splicing; implicated in rearranging and proofreading snRNA structure in catalytic activation of spliceosome; ortholog of human protein DHX16 (3, 4, 5)
Name Description Pre-mRNA Processing 1
Chromosomal Location
ChrXIV:646950 to 644320 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Genetic position: 8 cM
Gene Ontology Annotations All PRP2 GO evidence and references
  View Computational GO annotations for PRP2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 5 genes
Resources
Classical genetics
conditional
overexpression
Large-scale survey
null
reduction of function
Resources
95 total interaction(s) for 84 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 53
  • Affinity Capture-Western: 2
  • Protein-peptide: 5
  • Reconstituted Complex: 1
  • Two-hybrid: 5

Genetic Interactions
  • Dosage Rescue: 2
  • Negative Genetic: 1
  • Phenotypic Enhancement: 6
  • Positive Genetic: 14
  • Synthetic Growth Defect: 3
  • Synthetic Lethality: 2
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 876
Molecular Weight (Da) 99,813
Isoelectric Point (pI) 8.42
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIV:646950 to 644320 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Genetic position: 8 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1997-01-28
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2631 646950..644320 2011-02-03 1997-01-28
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005294
References cited on this page View Complete Literature Guide for PRP2
1) Vijayraghavan U, et al.  (1989) Isolation and characterization of pre-mRNA splicing mutants of Saccharomyces cerevisiae. Genes Dev 3(8):1206-16
2) Hartwell LH, et al.  (1970) Identification of ten genes that control ribosome formation in yeast. Mol Gen Genet 109(1):42-56
3) Kim SH and Lin RJ  (1996) Spliceosome activation by PRP2 ATPase prior to the first transesterification reaction of pre-mRNA splicing. Mol Cell Biol 16(12):6810-9
4) Gencheva M, et al.  (2010) Nuclear Retention of Unspliced Pre-mRNAs by Mutant DHX16/hPRP2, a Spliceosomal DEAH-box Protein. J Biol Chem 285(46):35624-32
5) Wlodaver AM and Staley JP  (2014) The DExD/H-box ATPase Prp2p destabilizes and proofreads the catalytic RNA core of the spliceosome. RNA 20(3):282-94