PRK1/YIL095W Summary Help

Standard Name PRK1 1
Systematic Name YIL095W
Alias PAK1
Feature Type ORF, Verified
Description Protein serine/threonine kinase; regulates the organization and function of the actin cytoskeleton and reduces endocytic ability of cell through the phosphorylation of the Pan1p-Sla1p-End3p protein complex; PRK1 has a paralog, ARK1, that arose from the whole genome duplication (1, 2)
Name Description p53 Regulatory Kinase 3
Chromosomal Location
ChrIX:183937 to 186369 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All PRK1 GO evidence and references
  View Computational GO annotations for PRK1
Molecular Function
Manually curated
High-throughput
Biological Process
Manually curated
High-throughput
Cellular Component
Manually curated
High-throughput
Regulators 1 genes
Resources
Classical genetics
null
overexpression
Large-scale survey
null
overexpression
Resources
198 total interaction(s) for 139 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 25
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 7
  • Biochemical Activity: 69
  • PCA: 1
  • Protein-peptide: 1
  • Reconstituted Complex: 1
  • Two-hybrid: 9

Genetic Interactions
  • Dosage Growth Defect: 13
  • Dosage Lethality: 1
  • Negative Genetic: 40
  • Phenotypic Enhancement: 4
  • Phenotypic Suppression: 3
  • Positive Genetic: 6
  • Synthetic Growth Defect: 5
  • Synthetic Haploinsufficiency: 1
  • Synthetic Lethality: 5
  • Synthetic Rescue: 5

Resources
Expression Summary
histogram
Resources
Length (a.a.) 810
Molecular Weight (Da) 91,031
Isoelectric Point (pI) 10.43
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIX:183937 to 186369 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1994-12-10
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2433 183937..186369 2011-02-03 1994-12-10
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001357
References cited on this page View Complete Literature Guide for PRK1
1) Zeng G and Cai M  (1999) Regulation of the actin cytoskeleton organization in yeast by a novel serine/threonine kinase Prk1p. J Cell Biol 144(1):71-82
2) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
3) Cope MJ, et al.  (1999) Novel protein kinases Ark1p and Prk1p associate with and regulate the cortical actin cytoskeleton in budding yeast. J Cell Biol 144(6):1203-18