PRE3/YJL001W Summary

PRE3 BASIC INFORMATION

Standard Name	PRE3
Systematic Name	YJL001W
Alias	CRL21
Feature Type	ORF, Verified
Description	Beta 1 subunit of the 20S proteasome, responsible for cleavage after acidic residues in peptides (1, 2)
Name Description	PRoteinase yscE

GO Annotations	All PRE3 GO evidence and references
	View Computational GO annotations for PRE3
Molecular Function
Manually curated	endopeptidase activity (TAS)
Biological Process
Manually curated	ascospore formation (TAS) response to stress (TAS) ubiquitin-dependent protein catabolic process (TAS)
Cellular Component
Manually curated	proteasome core complex, beta-subunit complex (IPI, TAS) proteasome storage granule (IDA)

Mutant Phenotype	All PRE3 Phenotype details and references
Classical genetics
conditional	heat sensitivity: increased
reduction of function	mutation frequency: increased
unspecified	resistance to cycloheximide: increased
Large-scale survey
null	inviable
repressible	mitochondrial morphology: abnormal

Interactions	PRE3 All interactions details and references
	121 total interaction(s) for 38 unique genes/features.
Physical Interactions	Affinity Capture-MS: 52 Affinity Capture-RNA: 1 Affinity Capture-Western: 2 Co-crystal Structure: 5 Co-purification: 1 Two-hybrid: 6
Genetic Interactions	Phenotypic Enhancement: 1 Synthetic Growth Defect: 18 Synthetic Rescue: 35

Sequence Information

ChrX:435156 to 435919 | |

Last Update

Coordinates: 2009-02-18 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..65	435156..435220	2009-02-18	1996-07-31
Intron	66..181	435221..435336	2009-02-18	1996-07-31
CDS	182..764	435337..435919	2009-02-18	1996-07-31

External Links	All Associated Seq \| E.C. \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| UniProtKB

Primary SGDID	S000003538

PRE3 RESOURCES

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SGD ORF map	GBrowse

Click on histogram for expression summary
Expression Summary

ADDITIONAL INFORMATION for PRE3

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for PRE3]

1)	Groll M, et al. (1999) The catalytic sites of 20S proteasomes and their role in subunit maturation: a mutational and crystallographic study. Proc Natl Acad Sci U S A 96(20):10976-83
2)	Jager S, et al. (1999) Proteasome beta-type subunits: unequal roles of propeptides in core particle maturation and a hierarchy of active site function. J Mol Biol 291(4):997-1013

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