PRE1/YER012W Summary

PRE1 BASIC INFORMATION

Standard Name	PRE1
Systematic Name	YER012W
Feature Type	ORF, Verified
Description	Beta 4 subunit of the 20S proteasome; localizes to the nucleus throughout the cell cycle (1, 2, 3, 4)
Name Description	PRoteinase yscE 3

GO Annotations	All PRE1 GO evidence and references
	View Computational GO annotations for PRE1
Molecular Function
Manually curated	endopeptidase activity (TAS)
Biological Process
Manually curated	ascospore formation (TAS) response to stress (TAS) ubiquitin-dependent protein catabolic process (IGI)
Cellular Component
Manually curated	nucleus (IDA) proteasome core complex, beta-subunit complex (IPI)

Mutant Phenotype	All PRE1 Phenotype details and references
Classical genetics
conditional	heat sensitivity: increased ubiquitinated proteins accumulation: increased resistance to canavanine: decreased resistance to cycloheximide: increased sporulation: absent
null	inviable
unspecified	resistance to cycloheximide: increased
Large-scale survey
conditional	heat sensitivity: increased
null	inviable
repressible	cell cycle progression in G2 phase: abnormal cytokinesis: abnormal mitochondrial morphology: abnormal

Interactions	PRE1 All interactions details and references
	286 total interaction(s) for 107 unique genes/features.
Physical Interactions	Affinity Capture-MS: 139 Affinity Capture-RNA: 1 Affinity Capture-Western: 64 Co-purification: 12 Two-hybrid: 3
Genetic Interactions	Dosage Lethality: 6 Phenotypic Enhancement: 7 Phenotypic Suppression: 1 Synthetic Growth Defect: 20 Synthetic Lethality: 2 Synthetic Rescue: 31

Sequence Information

ChrV:177834 to 178430 | |

Last Update

Coordinates: 1996-07-31 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..597	177834..178430	1996-07-31	1996-07-31

External Links	All Associated Seq \| E.C. \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| UniProtKB

Primary SGDID	S000000814

PRE1 RESOURCES

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SGD ORF map	GBrowse

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Expression Summary

ADDITIONAL INFORMATION for PRE1

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for PRE1]

1)	Russell SJ, et al. (1999) Subcellular localization, stoichiometry, and protein levels of 26 S proteasome subunits in yeast. J Biol Chem 274(31):21943-52
2)	Groll M, et al. (1999) The catalytic sites of 20S proteasomes and their role in subunit maturation: a mutational and crystallographic study. Proc Natl Acad Sci U S A 96(20):10976-83
3)	Heinemeyer W, et al. (1991) Proteinase yscE, the yeast proteasome/multicatalytic-multifunctional proteinase: mutants unravel its function in stress induced proteolysis and uncover its necessity for cell survival. EMBO J 10(3):555-62
4)	Hochstrasser M (1996) Ubiquitin-dependent protein degradation. Annu Rev Genet 30:405-39

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