PRB1/YEL060C Summary Help

Standard Name PRB1 1
Systematic Name YEL060C
Alias CVT1
Feature Type ORF, Verified
Description Vacuolar proteinase B (yscB) with H3 N-terminal endopeptidase activity; serine protease of the subtilisin family; involved in protein degradation in the vacuole and required for full protein degradation during sporulation; activity inhibited by Pbi2p; protein abundance increases in response to DNA replication stress; PRB1 has a paralog, YSP3, that arose from the whole genome duplication (2, 3, 4, 5, 6, 7, 8)
Name Description PRoteinase B 1
Chromosomal Location
ChrV:41953 to 40046 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Genetic position: -48 cM
Gene Ontology Annotations All PRB1 GO evidence and references
  View Computational GO annotations for PRB1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 17 genes
Classical genetics
reduction of function
Large-scale survey
81 total interaction(s) for 77 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 24
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 1
  • Biochemical Activity: 1
  • Two-hybrid: 2

Genetic Interactions
  • Negative Genetic: 40
  • Phenotypic Enhancement: 3
  • Positive Genetic: 1
  • Synthetic Growth Defect: 2
  • Synthetic Lethality: 3
  • Synthetic Rescue: 2

Expression Summary
Length (a.a.) 635
Molecular Weight (Da) 69,621
Isoelectric Point (pI) 6.37
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrV:41953 to 40046 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Genetic position: -48 cM
Last Update Coordinates: 1996-07-31 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1908 41953..40046 1996-07-31 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000786
References cited on this page View Complete Literature Guide for PRB1
1) Zubenko GS and Jones EW  (1979) Catabolite inactivation of gluconeogenic enzymes in mutants of yeast deficient in proteinase B. Proc Natl Acad Sci U S A 76(9):4581-5
2) Moehle CM, et al.  (1987) Protease B of the lysosomelike vacuole of the yeast Saccharomyces cerevisiae is homologous to the subtilisin family of serine proteases. Mol Cell Biol 7(12):4390-9
3) Schu P, et al.  (1991) The proteinase yscB inhibitor (PB12) gene of yeast and studies on the function of its protein product. Eur J Biochem 197(1):1-7
4) Zubenko GS and Jones EW  (1981) Protein degradation, meiosis and sporulation in proteinase-deficient mutants of Saccharomyces cerevisiae. Genetics 97(1):45-64
5) Teichert U, et al.  (1989) Lysosomal (vacuolar) proteinases of yeast are essential catalysts for protein degradation, differentiation, and cell survival. J Biol Chem 264(27):16037-45
6) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
7) Bauer R, et al.  (1976) Low and high pH form of cadmium carbonic anhydrase determined by nuclear quadrupole interaction. Biochemistry 15(2):334-42
8) Xue Y, et al.  (2014) PRB1 Is Required for Clipping of the Histone H3 N Terminal Tail in Saccharomyces cerevisiae. PLoS One 9(2):e90496