PPH3/YDR075W Summary Help

Standard Name PPH3 1
Systematic Name YDR075W
Feature Type ORF, Verified
Description Catalytic subunit of protein phosphatase PP4 complex; active complex is composed of Pph3p and Psy2p, with Psy4p apparently providing additional substrate specificity in some cases; regulates recovery from the DNA damage checkpoint and also the gene conversion- and single-strand annealing-mediated pathways of meiotic double-strand break repair; involved in activation of Gln3p to alleviate nitrogen catabolite repression; Pph3p and Psy2p localize to foci on meiotic chromosomes (2, 3, 4, 5, 6, 7, 8)
Name Description Protein PHosphatase
Chromosomal Location
ChrIV:597156 to 598082 | ORF Map | GBrowse
Gbrowse
Genetic position: 53 cM
Gene Ontology Annotations All PPH3 GO evidence and references
  View Computational GO annotations for PPH3
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulatory Role
Regulatory modules predicted: stressResponse (589, 559)
Mutant phenotypes All PPH3 Phenotype evidence and references
Classical genetics
null
Large-scale survey
null
overexpression
Resources
interactions All PPH3 Interaction evidence and references
267 total interaction(s) for 167 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 45
  • Affinity Capture-Western: 7
  • Biochemical Activity: 3
  • PCA: 1
  • Reconstituted Complex: 2
  • Two-hybrid: 4
Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Lethality: 1
  • Dosage Rescue: 1
  • Negative Genetic: 121
  • Phenotypic Enhancement: 6
  • Phenotypic Suppression: 12
  • Positive Genetic: 25
  • Synthetic Growth Defect: 17
  • Synthetic Lethality: 18
  • Synthetic Rescue: 3
Resources
Expression Summary
histogram
Resources
Protein Information All PPH3 Protein evidence and references
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:597156 to 598082 | ORF Map | GBrowse
SGD ORF map
Genetic position: 53 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..927 597156..598082 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002482
References cited on this page View Complete Literature Guide for PPH3
1) Ronne, H.  (1993) Personal Communication, Mortimer Map Edition 12
2) Bertram PG, et al.  (2000) Tripartite regulation of Gln3p by TOR, Ure2p, and phosphatases. J Biol Chem 275(46):35727-33
3) Hoffmann R, et al.  (1994) The Saccharomyces cerevisiae gene PPH3 encodes a protein phosphatase with properties different from PPX, PP1 and PP2A. Yeast 10(5):567-78
4) Keogh MC, et al.  (2006) A phosphatase complex that dephosphorylates gammaH2AX regulates DNA damage checkpoint recovery. Nature 439(7075):497-501
5) Hastie CJ, et al.  (2006) The Saccharomyces cerevisiae orthologue of the human protein phosphatase 4 core regulatory subunit R2 confers resistance to the anticancer drug cisplatin. FEBS J 273(14):3322-34
6) O'Neill BM, et al.  (2007) Pph3-Psy2 is a phosphatase complex required for Rad53 dephosphorylation and replication fork restart during recovery from DNA damage. Proc Natl Acad Sci U S A 104(22):9290-5
7) Falk JE, et al.  (2010) A Mec1- and PP4-dependent checkpoint couples centromere pairing to meiotic recombination. Dev Cell 19(4):599-611
8) Kim JA, et al.  (2011) Protein phosphatases pph3, ptc2, and ptc3 play redundant roles in DNA double-strand break repair by homologous recombination. Mol Cell Biol 31(3):507-16