PPH22/YDL188C Summary Help

Standard Name PPH22 1
Systematic Name YDL188C
Alias PPH2
Feature Type ORF, Verified
Description Catalytic subunit of protein phosphatase 2A (PP2A); functionally redundant with Pph21p; methylated at C terminus; forms alternate complexes with several regulatory subunits; involved in signal transduction and regulation of mitosis; protein abundance increases in response to DNA replication stress; PPH22 has a paralog, PPH21, that arose from the whole genome duplication (1, 2, 3, 4, 5, 6)
Name Description Protein PHosphatase 1
Chromosomal Location
ChrIV:124998 to 123865 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Genetic position: -102 cM
Gene Ontology Annotations All PPH22 GO evidence and references
  View Computational GO annotations for PPH22
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 2 genes
Resources
Classical genetics
null
overexpression
Large-scale survey
null
overexpression
unspecified
Resources
160 total interaction(s) for 97 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 69
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 12
  • PCA: 1
  • Reconstituted Complex: 2
  • Two-hybrid: 4

Genetic Interactions
  • Dosage Lethality: 4
  • Dosage Rescue: 6
  • Negative Genetic: 20
  • Phenotypic Enhancement: 6
  • Phenotypic Suppression: 1
  • Positive Genetic: 7
  • Synthetic Growth Defect: 8
  • Synthetic Haploinsufficiency: 1
  • Synthetic Lethality: 10
  • Synthetic Rescue: 8

Resources
Expression Summary
histogram
Resources
Length (a.a.) 377
Molecular Weight (Da) 43,047
Isoelectric Point (pI) 4.48
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:124998 to 123865 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Genetic position: -102 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1134 124998..123865 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002347
References cited on this page View Complete Literature Guide for PPH22
1) Sneddon AA, et al.  (1990) Saccharomyces cerevisiae protein phosphatase 2A performs an essential cellular function and is encoded by two genes. EMBO J 9(13):4339-46
2) Stark MJ  (1996) Yeast protein serine/threonine phosphatases: multiple roles and diverse regulation. Yeast 12(16):1647-75
3) Wei H, et al.  (2001) Carboxymethylation of the PP2A catalytic subunit in Saccharomyces cerevisiae is required for efficient interaction with the B-type subunits Cdc55p and Rts1p. J Biol Chem 276(2):1570-7
4) Zabrocki P, et al.  (2002) Protein phosphatase 2A on track for nutrient-induced signalling in yeast. Mol Microbiol 43(4):835-42
5) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
6) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76