PPH21/YDL134C Summary Help

Standard Name PPH21 1
Systematic Name YDL134C
Feature Type ORF, Verified
Description Catalytic subunit of protein phosphatase 2A (PP2A); functionally redundant with Pph22p; methylated at C terminus; forms alternate complexes with several regulatory subunits; involved in signal transduction and regulation of mitosis; forms nuclear foci upon DNA replication stress; PPH21 has a paralog, PPH22, that arose from the whole genome duplication (2, 3, 4, 5, 6, 7)
Name Description Protein PHosphatase 1
Chromosomal Location
ChrIV:220771 to 219662 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Genetic position: -66 cM
Gene Ontology Annotations All PPH21 GO evidence and references
  View Computational GO annotations for PPH21
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 1 genes
Classical genetics
Large-scale survey
221 total interaction(s) for 131 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 46
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 24
  • Co-purification: 2
  • PCA: 1
  • Reconstituted Complex: 2
  • Two-hybrid: 5

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Lethality: 4
  • Dosage Rescue: 5
  • Negative Genetic: 73
  • Phenotypic Enhancement: 7
  • Phenotypic Suppression: 4
  • Positive Genetic: 23
  • Synthetic Growth Defect: 8
  • Synthetic Haploinsufficiency: 1
  • Synthetic Lethality: 9
  • Synthetic Rescue: 5

Expression Summary
Length (a.a.) 369
Molecular Weight (Da) 41,938
Isoelectric Point (pI) 4.46
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrIV:220771 to 219662 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Genetic position: -66 cM
Last Update Coordinates: 1996-07-31 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1110 220771..219662 1996-07-31 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002292
References cited on this page View Complete Literature Guide for PPH21
1) Ronne H, et al.  (1991) Protein phosphatase 2A in Saccharomyces cerevisiae: effects on cell growth and bud morphogenesis. Mol Cell Biol 11(10):4876-84
2) Lin FC and Arndt KT  (1995) The role of Saccharomyces cerevisiae type 2A phosphatase in the actin cytoskeleton and in entry into mitosis. EMBO J 14(12):2745-59
3) Sneddon AA, et al.  (1990) Saccharomyces cerevisiae protein phosphatase 2A performs an essential cellular function and is encoded by two genes. EMBO J 9(13):4339-46
4) Wei H, et al.  (2001) Carboxymethylation of the PP2A catalytic subunit in Saccharomyces cerevisiae is required for efficient interaction with the B-type subunits Cdc55p and Rts1p. J Biol Chem 276(2):1570-7
5) Zabrocki P, et al.  (2002) Protein phosphatase 2A on track for nutrient-induced signalling in yeast. Mol Microbiol 43(4):835-42
6) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
7) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76