PPH21/YDL134C Summary

PPH21 BASIC INFORMATION

Standard Name	PPH21
Systematic Name	YDL134C
Alias	PPH1
Feature Type	ORF, Verified
Description	Catalytic subunit of protein phosphatase 2A (PP2A), functionally redundant with Pph22p; methylated at C terminus; forms alternate complexes with several regulatory subunits; involved in signal transduction and regulation of mitosis (1, 2, 3, 4)
Name Description	Protein PHosphatase

GO Annotations	All PPH21 GO evidence and references
	View Computational GO annotations for PPH21
Molecular Function
Manually curated	protein serine/threonine phosphatase activity (IMP)
Biological Process
Manually curated	actin filament organization (TAS) budding cell bud growth (TAS) G1/S transition of mitotic cell cycle (IGI) mitotic cell cycle spindle assembly checkpoint (IPI) protein amino acid dephosphorylation (TAS) regulation of translation (IPI)
Cellular Component
Manually curated	protein phosphatase type 2A complex (IDA)

Mutant Phenotype	All PPH21 Phenotype details and references
Classical genetics
null	viability: increased
overexpression	budding index: abnormal cell cycle progression in G1 phase: abnormal cell size: increased cellular morphology: abnormal
Large-scale survey
null	competitive fitness: decreased resistance to 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid: decreased viable
overexpression	vegetative growth: decreased

Interactions	PPH21 All interactions details and references
	102 total interaction(s) for 53 unique genes/features.
Physical Interactions	Affinity Capture-MS: 35 Affinity Capture-Western: 16 Co-purification: 2 Reconstituted Complex: 2 Two-hybrid: 4
Genetic Interactions	Dosage Lethality: 2 Dosage Rescue: 4 Phenotypic Enhancement: 14 Phenotypic Suppression: 11 Synthetic Growth Defect: 4 Synthetic Haploinsufficiency: 1 Synthetic Lethality: 5 Synthetic Rescue: 2

Sequence Information

ChrIV:220771 to 219662 | |

Note: this feature is encoded on the Crick strand.

Genetic position: -66 cM

Last Update

Coordinates: 1996-07-31 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..1110	220771..219662	1996-07-31	1996-07-31

External Links	All Associated Seq \| E.C. \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| UniProtKB

Primary SGDID	S000002292

PPH21 RESOURCES

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SGD ORF map	GBrowse

Click on histogram for expression summary
Expression Summary

ADDITIONAL INFORMATION for PPH21

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for PPH21]

1)	Lin FC and Arndt KT (1995) The role of Saccharomyces cerevisiae type 2A phosphatase in the actin cytoskeleton and in entry into mitosis. EMBO J 14(12):2745-59
2)	Sneddon AA, et al. (1990) Saccharomyces cerevisiae protein phosphatase 2A performs an essential cellular function and is encoded by two genes. EMBO J 9(13):4339-46
3)	Wei H, et al. (2001) Carboxymethylation of the PP2A catalytic subunit in Saccharomyces cerevisiae is required for efficient interaction with the B-type subunits Cdc55p and Rts1p. J Biol Chem 276(2):1570-7
4)	Zabrocki P, et al. (2002) Protein phosphatase 2A on track for nutrient-induced signalling in yeast. Mol Microbiol 43(4):835-42

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