PPH21/YDL134C Summary

PPH21/YDL134C Summary

Standard Name	PPH21
Systematic Name	YDL134C
Alias	PPH1
Feature Type	ORF, Verified
Description	Catalytic subunit of protein phosphatase 2A (PP2A); functionally redundant with Pph22p; methylated at C terminus; forms alternate complexes with several regulatory subunits; involved in signal transduction and regulation of mitosis; forms nuclear foci upon DNA replication stress; PPH21 has a paralog, PPH22, that arose from the whole genome duplication (1, 2, 3, 4, 5, 6)
Name Description	Protein PHosphatase

Chromosomal Location	ChrIV:220771 to 219662 \| ORF Map \| GBrowse
	Note: this feature is encoded on the Crick strand.

	Genetic position: -66 cM

Gene Ontology Annotations All PPH21 GO evidence and references

	View Computational GO annotations for PPH21
Molecular Function
Manually curated	protein serine/threonine phosphatase activity (IMP)
Biological Process
Manually curated	actin filament organization (TAS) budding cell bud growth (TAS) G1/S transition of mitotic cell cycle (IGI) mitotic spindle assembly checkpoint (IPI) protein dephosphorylation (TAS) regulation of translation (IPI)
Cellular Component
Manually curated	protein phosphatase type 2A complex (IDA)
High-throughput	cytoplasm (IDA) nucleus (IDA)

Mutant phenotypes All PPH21 Phenotype evidence and references

Classical genetics
null	Hos2p-GFP distribution: abnormal viability: increased
overexpression	budding index: abnormal cell cycle progression in G1 phase: abnormal cell size: increased cellular morphology: abnormal
Large-scale survey
null	competitive fitness: decreased haploproficient lipid particle morphology: abnormal Fus-Mid-GFP distribution: abnormal resistance to acetic acid: increased resistance to caffeine: increased resistance to 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid: decreased resistance to camptothecin: decreased resistance to cycloheximide: decreased resistance to fenpropimorph: decreased resistance to fluconazole: decreased resistance to methyl methanesulfonate: decreased resistance to bleomycin: decreased sporulation efficiency: increased starvation resistance: decreased viable
overexpression	cell cycle progression: abnormal vegetative growth: decreased
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All PPH21 Interaction evidence and references

	215 total interaction(s) for 129 unique genes/features.
Physical Interactions	Affinity Capture-MS: 45 Affinity Capture-Western: 24 Co-purification: 2 PCA: 1 Reconstituted Complex: 2 Two-hybrid: 5
Genetic Interactions	Dosage Growth Defect: 1 Dosage Lethality: 4 Dosage Rescue: 5 Negative Genetic: 70 Phenotypic Enhancement: 7 Phenotypic Suppression: 4 Positive Genetic: 23 Synthetic Growth Defect: 8 Synthetic Haploinsufficiency: 1 Synthetic Lethality: 9 Synthetic Rescue: 4
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder \| YeastRC Two-Hybrid

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All PPH21 Protein evidence and references

Localization	YeastRC \| Organelle DB (UMich) \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| AspGD Homologs \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrIV:220771 to 219662 | |

Note: this feature is encoded on the Crick strand.

SGD ORF map

: -66 cM

Last Update

Coordinates: 1996-07-31 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..1110	220771..219662	1996-07-31	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| E.C. \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000002292

References cited on this page View Complete Literature Guide for PPH21

1)	Lin FC and Arndt KT (1995) The role of Saccharomyces cerevisiae type 2A phosphatase in the actin cytoskeleton and in entry into mitosis. EMBO J 14(12):2745-59
2)	Sneddon AA, et al. (1990) Saccharomyces cerevisiae protein phosphatase 2A performs an essential cellular function and is encoded by two genes. EMBO J 9(13):4339-46
3)	Wei H, et al. (2001) Carboxymethylation of the PP2A catalytic subunit in Saccharomyces cerevisiae is required for efficient interaction with the B-type subunits Cdc55p and Rts1p. J Biol Chem 276(2):1570-7
4)	Zabrocki P, et al. (2002) Protein phosphatase 2A on track for nutrient-induced signalling in yeast. Mol Microbiol 43(4):835-42
5)	Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
6)	Tkach JM, et al. (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76