POR1/YNL055C Summary

POR1/YNL055C Summary

Standard Name	POR1
Systematic Name	YNL055C
Alias	OMP2
Feature Type	ORF, Verified
Description	Mitochondrial porin (voltage-dependent anion channel); outer membrane protein required for maintenance of mitochondrial osmotic stability and mitochondrial membrane permeability; couples the glutathione pools of the intermembrane space (IMS) and the cytosol; phosphorylated; protein abundance increases in response to DNA replication stress; POR1 has a paralog, POR2, that arose from the whole genome duplication (1, 2, 3, 4, 5, 6) Also known as: YVDAC1 , VDAC 7
Name Description	PORin

Chromosomal Location	ChrXIV:518845 to 517994 \| ORF Map \| GBrowse
	Note: this feature is encoded on the Crick strand.

Gene Ontology Annotations All POR1 GO evidence and references

	View Computational GO annotations for POR1
Molecular Function
Manually curated	voltage-gated anion channel activity (IDA)
Biological Process
Manually curated	apoptotic process (IMP) cell redox homeostasis (IMP) DNA transport (IMP) ion transport (IDA) mitochondrion organization (IMP)
Cellular Component
Manually curated	integral to mitochondrial outer membrane (IDA) mitochondrial outer membrane (IDA) mitochondrion (IPI)
High-throughput	cytoplasm (IDA) mitochondrial outer membrane (IDA) mitochondrion (IDA)

Mutant phenotypes All POR1 Phenotype evidence and references

Classical genetics
null	acid pH resistance: decreased apoptosis: decreased oxidative stress resistance: decreased Sod1p activity: decreased redox state: abnormal resistance to sirolimus: increased respiratory growth: absent respiratory metabolism: decreased survival rate in stationary phase: decreased viability: increased viability: decreased
Large-scale survey
null	acid pH resistance: decreased cell size: decreased competitive fitness: decreased haploinsufficient heat sensitivity: increased mitochondrial morphology: abnormal resistance to doxorubicin: decreased resistance to sorbate: decreased resistance to hydroxyurea: increased resistance to ethanol: decreased resistance to elesclomol-Cu: decreased stress resistance: increased toxin resistance: decreased
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All POR1 Interaction evidence and references

	241 total interaction(s) for 188 unique genes/features.
Physical Interactions	Affinity Capture-MS: 72 Affinity Capture-RNA: 6 Affinity Capture-Western: 10 Co-fractionation: 3 Co-localization: 1 Co-purification: 1 PCA: 1 Two-hybrid: 2
Genetic Interactions	Dosage Lethality: 1 Dosage Rescue: 1 Negative Genetic: 114 Phenotypic Enhancement: 3 Phenotypic Suppression: 1 Positive Genetic: 14 Synthetic Growth Defect: 7 Synthetic Lethality: 4
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder \| YeastRC Mass Spec

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All POR1 Protein evidence and references

Localization	YeastRC \| Organelle DB (UMich) \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| AspGD Homologs \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrXIV:518845 to 517994 | |

Note: this feature is encoded on the Crick strand.

SGD ORF map

Last Update

Coordinates: 2011-02-03 | Sequence: 1997-01-28

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..852	518845..517994	2011-02-03	1997-01-28

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| TCDB \| UniProtKB

Primary SGDID	S000005000

References cited on this page View Complete Literature Guide for POR1

1)	Lee AC, et al. (1998) The role of yeast VDAC genes on the permeability of the mitochondrial outer membrane. J Membr Biol 161(2):173-81
2)	Sanchez NS, et al. (2001) Requirements of Cyc2p and the porin, Por1p, for ionic stability and mitochondrial integrity in Saccharomyces cerevisiae. Arch Biochem Biophys 392(2):326-32
3)	Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
4)	Reinders J, et al. (2007) Profiling phosphoproteins of yeast mitochondria reveals a role of phosphorylation in assembly of the ATP synthase. Mol Cell Proteomics 6(11):1896-906
5)	Tkach JM, et al. (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
6)	Kojer K, et al. (2012) Glutathione redox potential in the mitochondrial intermembrane space is linked to the cytosol and impacts the Mia40 redox state. EMBO J 31(14):3169-82
7)	Forte M, et al. (1987) Molecular genetics of the VDAC ion channel: structural model and sequence analysis. J Bioenerg Biomembr 19(4):341-50