POL31/YJR006W Summary Help

Standard Name POL31 1
Systematic Name YJR006W
Alias HUS2 , SDP5 2 , HYS2 3
Feature Type ORF, Verified
Description Subunit of DNA polymerase delta (polymerase III); essential for cell viability; involved in DNA replication and DNA repair; forms a complex with Rev3p, Rev7p and Pol32p; relocalizes to the cytosol in response to hypoxia (1, 4, 5, 6, 7)
Name Description POLymerase 1
Chromosomal Location
ChrX:449202 to 450665 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All POL31 GO evidence and references
  View Computational GO annotations for POL31
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 11 genes
Resources
Classical genetics
conditional
null
Large-scale survey
conditional
null
Resources
78 total interaction(s) for 41 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 5
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 7
  • Co-purification: 2
  • Reconstituted Complex: 7
  • Two-hybrid: 4

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Lethality: 3
  • Dosage Rescue: 2
  • Negative Genetic: 18
  • Phenotypic Enhancement: 1
  • Positive Genetic: 3
  • Synthetic Growth Defect: 7
  • Synthetic Lethality: 10
  • Synthetic Rescue: 7

Resources
Expression Summary
histogram
Resources
Length (a.a.) 487
Molecular Weight (Da) 55,295
Isoelectric Point (pI) 4.66
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrX:449202 to 450665 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1464 449202..450665 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003766
References cited on this page View Complete Literature Guide for POL31
1) Gerik KJ, et al.  (1998) Characterization of the two small subunits of Saccharomyces cerevisiae DNA polymerase delta. J Biol Chem 273(31):19747-55
2) Giot L, et al.  (1995) Suppressors of thermosensitive mutations in the DNA polymerase delta gene of Saccharomyces cerevisiae. Mol Gen Genet 246(2):212-22
3) Sugimoto K, et al.  (1995) HYS2, an essential gene required for DNA replication in Saccharomyces cerevisiae. Nucleic Acids Res 23(17):3493-500
4) Giot L, et al.  (1997) Involvement of the yeast DNA polymerase delta in DNA repair in vivo. Genetics 146(4):1239-51
5) Hashimoto K, et al.  (1998) The second subunit of DNA polymerase III (delta) is encoded by the HYS2 gene in Saccharomyces cerevisiae. Nucleic Acids Res 26(2):477-85
6) Ghosh Dastidar R, et al.  (2012) The nuclear localization of SWI/SNF proteins is subjected to oxygen regulation. Cell Biosci 2(1):30
7) Makarova AV, et al.  (2012) A four-subunit DNA polymerase ? complex containing Pol d accessory subunits is essential for PCNA-mediated mutagenesis. Nucleic Acids Res 40(22):11618-26