POL3/YDL102W Summary

POL3/YDL102W Summary

Standard Name	POL3 1 (see Nomenclature conflict Note)
Systematic Name	YDL102W
Alias	HPR6 , TEX1 2 , CDC2 3
Feature Type	ORF, Verified
Description	Catalytic subunit of DNA polymerase delta; required for chromosomal DNA replication during mitosis and meiosis, intragenic recombination, repair of double strand DNA breaks, and DNA replication during nucleotide excision repair (NER) (4)
Name Description	POLymerase 1

Chromosomal Location	ChrIV:276872 to 280165 \| ORF Map \| GBrowse

	Genetic position: -55 cM

Gene Ontology Annotations All POL3 GO evidence and references

	View Computational GO annotations for POL3
Molecular Function
Manually curated	DNA-directed DNA polymerase activity (IDA, IMP) single-stranded DNA specific 3'-5' exodeoxyribonuclease activity (TAS)
Biological Process
Manually curated	DNA replication (IMP) DNA replication, removal of RNA primer (IDA) maintenance of fidelity involved in DNA-dependent DNA replication (IGI) RNA-dependent DNA replication (IDA)
Cellular Component
Manually curated	delta DNA polymerase complex (TAS) replication fork (IDA)

Mutant phenotypes All POL3 Phenotype evidence and references

Classical genetics
conditional	cell cycle progression: arrested cell cycle progression in S phase: arrested cellular morphology: abnormal heat sensitivity: increased
reduction of function	mutation frequency: increased
unspecified	mutation frequency: increased resistance to phosphonoacetic acid: decreased
Large-scale survey
conditional	chromosome/plasmid maintenance: abnormal colony sectoring: increased resistance to hydroxyurea: decreased resistance to methyl methanesulfonate: decreased
null	haploproficient inviable resistance to sulfanilamide: decreased
reduction of function	competitive fitness: normal
Resources	PROPHECY \| PhenoM \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All POL3 Interaction evidence and references

	293 total interaction(s) for 172 unique genes/features.
Physical Interactions	Affinity Capture-MS: 7 Affinity Capture-Western: 3 Biochemical Activity: 1 Co-purification: 2 Reconstituted Complex: 5 Two-hybrid: 2
Genetic Interactions	Dosage Growth Defect: 2 Dosage Lethality: 5 Dosage Rescue: 4 Negative Genetic: 99 Phenotypic Enhancement: 24 Phenotypic Suppression: 14 Positive Genetic: 10 Synthetic Growth Defect: 18 Synthetic Lethality: 89 Synthetic Rescue: 8
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All POL3 Protein evidence and references

Localization	YeastRC \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrIV:276872 to 280165 | |

SGD ORF map

: -55 cM

Last Update

Coordinates: 2004-02-11 | Sequence: 2011-02-03

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..3294	276872..280165	2004-02-11	2011-02-03

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| E.C. \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000002260

NOMENCLATURE CONFLICT NOTE

Name	Relevance	Description
CDC28	Nomenclature conflict	CDC28/YBR160W encodes the catalytic subunit of the main cyclin-dependent protein kinase, which is the pombe Cdc2 ortholog. Do not confuse CDC28 with the cerevisiae POL3/YDL102W gene (originally named CDC2), which encodes a subunit of DNA polymerase III.
TEX1	Nomenclature conflict	The TEX1 gene name has been used by Gordenin et al., 1991 and Delgado et al., 1982 to describe POL3/YDL102W, a subunit of DNA polymerase III, and by Strasser et al., 2002 to describe TEX1/YNL253W, a component of the transcription export complex.
CDC6	Nomenclature conflict	CDC6/YJL194W encodes an ATPase required for DNA replication and is homologous to pombe cdc18. Do not confuse CDC6/YJL194W with the S. pombe cdc6 gene, which encodes a DNA polymerase delta subunit and is similar to the S. cerevisiae POL3/YDL102W.

References cited on this page View Complete Literature Guide for POL3

1)	Burgers PM, et al. (1990) Revised nomenclature for eukaryotic DNA polymerases. Eur J Biochem 191(3):617-8
2)	Delgado MA, et al. (1982) Genetic and biochemical study of threonine-overproducing mutants of Saccharomyces cerevisiae. Mol Cell Biol 2(7):731-6
3)	Hartwell LH, et al. (1970) Genetic control of the cell-division cycle in yeast. I. Detection of mutants. Proc Natl Acad Sci U S A 66(2):352-9
4)	Chanet R and Heude M (2003) Characterization of mutations that are synthetic lethal with pol3-13, a mutated allele of DNA polymerase delta in Saccharomyces cerevisiae. Curr Genet 43(5):337-50