POL2/YNL262W Summary

POL2 BASIC INFORMATION

Standard Name	POL2 1
Systematic Name	YNL262W
Alias	DUN2 2
Feature Type	ORF, Verified
Description	Catalytic subunit of DNA polymerase (II) epsilon, a chromosomal DNA replication polymerase that exhibits processivity and proofreading exonuclease activity; also involved in DNA synthesis during DNA repair; interacts extensively with Mrc1p (3, 4, 5)
Name Description	POLymerase 1

GO Annotations	All POL2 GO evidence and references
	View Computational GO annotations for POL2
Molecular Function
Manually curated	DNA-directed DNA polymerase activity (IMP) contributes_to double-stranded DNA binding (IDA)
Biological Process
Manually curated	chromatin silencing at telomere (IMP) DNA replication during S phase (IEP, IMP) DNA synthesis during DNA repair (IMP) double-strand break repair via nonhomologous end joining (IGI, IMP) lagging strand elongation (TAS) leading strand elongation (TAS) mismatch repair (TAS) mitotic sister chromatid cohesion (IMP) nucleotide-excision repair (TAS)
Cellular Component
Manually curated	epsilon DNA polymerase complex (IDA) replication fork (IDA)

Mutant Phenotype	All POL2 Phenotype details and references
Classical genetics
conditional	cell cycle progression in S phase: arrested heat sensitivity: increased resistance to hydroxyurea: decreased resistance to camptothecin: decreased resistance to methyl methanesulfonate: decreased
Large-scale survey
null	inviable
repressible	cell cycle progression in S phase: abnormal

Interactions	POL2 All interactions details and references
	135 total interaction(s) for 52 unique genes/features.
Physical Interactions	Affinity Capture-MS: 36 Affinity Capture-RNA: 1 Affinity Capture-Western: 14 Co-crystal Structure: 1 Co-fractionation: 3 Co-purification: 12 Reconstituted Complex: 4 Two-hybrid: 20
Genetic Interactions	Dosage Rescue: 9 Phenotypic Enhancement: 15 Phenotypic Suppression: 5 Synthetic Growth Defect: 4 Synthetic Lethality: 11

Sequence Information

ChrXIV:148213 to 154881 | |

Genetic position: -170 cM

Last Update

Coordinates: 2005-11-07 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..6669	148213..154881	2005-11-07	1996-07-31

External Links	All Associated Seq \| E.C. \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| UniProtKB

Primary SGDID	S000005206

POL2 RESOURCES

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SGD ORF map	GBrowse

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Expression Summary

ADDITIONAL INFORMATION for POL2

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for POL2]

1)	Morrison A, et al. (1990) A third essential DNA polymerase in S. cerevisiae. Cell 62(6):1143-51
2)	Humphrey T and Enoch T (1995) Cell-cycle checkpoints. Keeping mitosis in check. Curr Biol 5(4):376-9
3)	Burgers PM (1998) Eukaryotic DNA polymerases in DNA replication and DNA repair. Chromosoma 107(4):218-27
4)	Shimizu K, et al. (2002) Fidelity of DNA polymerase epsilon holoenzyme from budding yeast Saccharomyces cerevisiae. J Biol Chem 277(40):37422-9
5)	Lou H, et al. (2008) Mrc1 and DNA polymerase epsilon function together in linking DNA replication and the S phase checkpoint. Mol Cell 32(1):106-17

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