POL2/YNL262W Summary Help

Standard Name POL2 1
Systematic Name YNL262W
Alias DUN2 2
Feature Type ORF, Verified
Description Catalytic subunit of DNA polymerase (II) epsilon; a chromosomal DNA replication polymerase that exhibits processivity and proofreading exonuclease activity; participates in leading-strand synthesis during DNA replication; also involved in DNA synthesis during DNA repair; interacts extensively with Mrc1p (3, 4, 5, 6)
Name Description POLymerase 1
Chromosomal Location
ChrXIV:148212 to 154880 | ORF Map | GBrowse
Gbrowse
Genetic position: -170 cM
Gene Ontology Annotations All POL2 GO evidence and references
  View Computational GO annotations for POL2
Molecular Function
Manually curated
High-throughput
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 5 genes
Resources
Classical genetics
conditional
reduction of function
repressible
Large-scale survey
null
reduction of function
repressible
Resources
196 total interaction(s) for 78 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 42
  • Affinity Capture-RNA: 4
  • Affinity Capture-Western: 23
  • Co-crystal Structure: 1
  • Co-fractionation: 3
  • Co-purification: 12
  • Reconstituted Complex: 4
  • Two-hybrid: 20

Genetic Interactions
  • Dosage Rescue: 11
  • Negative Genetic: 1
  • Phenotypic Enhancement: 30
  • Phenotypic Suppression: 8
  • Positive Genetic: 1
  • Synthetic Growth Defect: 13
  • Synthetic Lethality: 22
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 2,222
Molecular Weight (Da) 255,669
Isoelectric Point (pI) 6.92
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIV:148212 to 154880 | ORF Map | GBrowse
SGD ORF map
Genetic position: -170 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..6669 148212..154880 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005206
References cited on this page View Complete Literature Guide for POL2
1) Morrison A, et al.  (1990) A third essential DNA polymerase in S. cerevisiae. Cell 62(6):1143-51
2) Navas TA, et al.  (1995) DNA polymerase epsilon links the DNA replication machinery to the S phase checkpoint. Cell 80(1):29-39
3) Burgers PM  (1998) Eukaryotic DNA polymerases in DNA replication and DNA repair. Chromosoma 107(4):218-27
4) Shimizu K, et al.  (2002) Fidelity of DNA polymerase epsilon holoenzyme from budding yeast Saccharomyces cerevisiae. J Biol Chem 277(40):37422-9
5) Lou H, et al.  (2008) Mrc1 and DNA polymerase epsilon function together in linking DNA replication and the S phase checkpoint. Mol Cell 32(1):106-17
6) Hogg M, et al.  (2014) Structural basis for processive DNA synthesis by yeast DNA polymerase ?. Nat Struct Mol Biol 21(1):49-55