PMI40/YER003C Summary

Standard Name	PMI40 1
Systematic Name	YER003C
Feature Type	ORF, Verified
Description	Mannose-6-phosphate isomerase, catalyzes the interconversion of fructose-6-P and mannose-6-P; required for early steps in protein mannosylation (1 and see Summary Paragraph) Also known as: PMI
Name Description	PhosphoMannose Isomerase 1

Chromosomal Location	ChrV:159118 to 157736 \| ORF Map \| GBrowse
	Note: this feature is encoded on the Crick strand.

Gene Ontology Annotations All PMI40 GO evidence and references

	View Computational GO annotations for PMI40
Molecular Function
Manually curated	mannose-6-phosphate isomerase activity (IDA)
Biological Process
Manually curated	cell wall mannoprotein biosynthetic process (IMP) GDP-mannose biosynthetic process (IMP) protein glycosylation (IMP)
Cellular Component
High-throughput	cytoplasm (IDA) nucleus (IDA)

Pathways	dolichyl phosphate D-mannose biosynthesis mannose degradation

Mutant phenotypes All PMI40 Phenotype evidence and references

Classical genetics
conditional	cell shape: abnormal flocculation: increased heat sensitivity: increased protein secretion: abnormal
null	auxotrophy utilization of carbon source: abnormal
Large-scale survey
conditional	heat sensitivity: increased
null	inviable starvation resistance: decreased
reduction of function	competitive fitness: increased
repressible	chromosome/plasmid maintenance: decreased
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All PMI40 Interaction evidence and references

	16 total interaction(s) for 16 unique genes/features.
Physical Interactions	Affinity Capture-MS: 6 Affinity Capture-RNA: 2 Two-hybrid: 1
Genetic Interactions	Synthetic Growth Defect: 5 Synthetic Lethality: 2
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All PMI40 Protein evidence and references

Localization	YeastRC \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| AspGD Homologs \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrV:159118 to 157736 | |

Note: this feature is encoded on the Crick strand.

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..31	159118..159088	2011-02-03	1996-07-31
Intron	32..124	159087..158995	2011-02-03	1996-07-31
CDS	125..1383	158994..157736	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| E.C. \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000000805

SUMMARY PARAGRAPH for PMI40

PMI40 encodes phosphomannose isomerase (EC 5.3.1.8), which catalyzes the isomerization between mannose-6-phosphate (M6P) and fructose-6-phosphate; M6P is a substrate for Sec53p and is used as a source of mannose for N-linked and O-linked glycosylation of proteins and GPI anchoring. Deletion or heat-inactivation of Pmi40p results in mannose auxotrophy, clumping, abnormal morphology, and defective secretion and cell-surface presentation of glycoproteins (2); unlike sec53, the pmi40 phenotype is suppressed if the growth medium is supplemented with mannose, presumably since mannose is imported into the cell as M6P, bypassing the need for PMI40 (1). Pmi40p is a zinc-dependent metalloenzyme; zinc, cadmium, and mercury are competitive inhibitors (3).

Mutations in the human homolog, PMI1 (OMIM), cause the congenital disorder of glycosylation CDG-Ib (OMIM). The human (4), Candida albicans (5), and Hansenula polymorpha (6) homologs all complement the pmi40 mutation. Deletion of the homolog MAN1 disrupts the pathogenicity of Cryptococcus neoformans (7).

Last updated: 2005-07-01

References cited on this page View Complete Literature Guide for PMI40

1)	Smith DJ, et al. (1992) PMI40, an intron-containing gene required for early steps in yeast mannosylation. Mol Cell Biol 12(7):2924-30
2)	Payton MA, et al. (1991) A novel Saccharomyces cerevisiae secretory mutant possesses a thermolabile phosphomannose isomerase. J Bacteriol 173(6):2006-10
3)	Wells TN, et al. (1993) Phosphomannose isomerase from Saccharomyces cerevisiae contains two inhibitory metal ion binding sites. Biochemistry 32(5):1294-301
4)	Proudfoot AE, et al. (1994) Purification, cDNA cloning and heterologous expression of human phosphomannose isomerase. Eur J Biochem 219(1-2):415-23
5)	Smith DJ, et al. (1995) Cloning and heterologous expression of the Candida albicans gene PMI 1 encoding phosphomannose isomerase. Yeast 11(4):301-10
6)	Kim MW, et al. (2002) Sequencing and functional analysis of the Hansenula polymorpha genomic fragment containing the YPT1 and PMI40 genes. Yeast 19(10):863-71
7)	Wills EA, et al. (2001) Identification and characterization of the Cryptococcus neoformans phosphomannose isomerase-encoding gene, MAN1, and its impact on pathogenicity. Mol Microbiol 40(3):610-20