PMI40/YER003C Summary Help

Standard Name PMI40 1
Systematic Name YER003C
Feature Type ORF, Verified
Description Mannose-6-phosphate isomerase; catalyzes the interconversion of fructose-6-P and mannose-6-P; required for early steps in protein mannosylation (1 and see Summary Paragraph)
Also known as: PMI
Name Description PhosphoMannose Isomerase 1
Chromosomal Location
ChrV:159118 to 157736 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All PMI40 GO evidence and references
  View Computational GO annotations for PMI40
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
High-throughput
Regulators 5 genes
Resources
Pathways
Classical genetics
conditional
null
Large-scale survey
conditional
null
reduction of function
repressible
Resources
20 total interaction(s) for 18 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 8
  • Affinity Capture-RNA: 2
  • Two-hybrid: 1

Genetic Interactions
  • Negative Genetic: 2
  • Synthetic Growth Defect: 5
  • Synthetic Lethality: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 429
Molecular Weight (Da) 48,188
Isoelectric Point (pI) 5.86
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrV:159118 to 157736 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..31 159118..159088 2011-02-03 1996-07-31
Intron 32..124 159087..158995 2011-02-03 1996-07-31
CDS 125..1383 158994..157736 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000805
SUMMARY PARAGRAPH for PMI40

PMI40 encodes phosphomannose isomerase (EC 5.3.1.8), which catalyzes the isomerization between mannose-6-phosphate (M6P) and fructose-6-phosphate; M6P is a substrate for Sec53p and is used as a source of mannose for N-linked and O-linked glycosylation of proteins and GPI anchoring. Deletion or heat-inactivation of Pmi40p results in mannose auxotrophy, clumping, abnormal morphology, and defective secretion and cell-surface presentation of glycoproteins (2); unlike sec53, the pmi40 phenotype is suppressed if the growth medium is supplemented with mannose, presumably since mannose is imported into the cell as M6P, bypassing the need for PMI40 (1). Pmi40p is a zinc-dependent metalloenzyme; zinc, cadmium, and mercury are competitive inhibitors (3).

Mutations in the human homolog, PMI1 (OMIM), cause the congenital disorder of glycosylation CDG-Ib (OMIM). The human (4), Candida albicans (5), and Hansenula polymorpha (6) homologs all complement the pmi40 mutation. Deletion of the homolog MAN1 disrupts the pathogenicity of Cryptococcus neoformans (7).

Last updated: 2005-07-01 Contact SGD

References cited on this page View Complete Literature Guide for PMI40
1) Smith DJ, et al.  (1992) PMI40, an intron-containing gene required for early steps in yeast mannosylation. Mol Cell Biol 12(7):2924-30
2) Payton MA, et al.  (1991) A novel Saccharomyces cerevisiae secretory mutant possesses a thermolabile phosphomannose isomerase. J Bacteriol 173(6):2006-10
3) Wells TN, et al.  (1993) Phosphomannose isomerase from Saccharomyces cerevisiae contains two inhibitory metal ion binding sites. Biochemistry 32(5):1294-301
4) Proudfoot AE, et al.  (1994) Purification, cDNA cloning and heterologous expression of human phosphomannose isomerase. Eur J Biochem 219(1-2):415-23
5) Smith DJ, et al.  (1995) Cloning and heterologous expression of the Candida albicans gene PMI 1 encoding phosphomannose isomerase. Yeast 11(4):301-10
6) Woong Kim M, et al.  (2002) Sequencing and functional analysis of the Hansenula polymorpha genomic fragment containing the YPT1 and PMI40 genes. Yeast 19(10):863-71
7) Wills EA, et al.  (2001) Identification and characterization of the Cryptococcus neoformans phosphomannose isomerase-encoding gene, MAN1, and its impact on pathogenicity. Mol Microbiol 40(3):610-20