PLP2/YOR281C Summary

Standard Name	PLP2 1
Systematic Name	YOR281C
Feature Type	ORF, Verified
Description	Essential protein that interacts with the CCT (chaperonin containing TCP-1) complex to stimulate actin folding; has similarity to phosducins; null mutant lethality is complemented by mouse phosducin-like protein MgcPhLP (1, 2, 3, 4 and see Summary Paragraph)
Name Description	Phosducin-Like Protein 1

Chromosomal Location	ChrXV:847129 to 846269 \| ORF Map \| GBrowse
	Note: this feature is encoded on the Crick strand.

Gene Ontology Annotations All PLP2 GO evidence and references

	View Computational GO annotations for PLP2
Molecular Function
Manually curated	actin binding (IDA, IMP) G-protein beta/gamma-subunit complex binding (IDA)
Biological Process
Manually curated	positive regulation of transcription from RNA polymerase II promoter by pheromones (IMP, IPI) protein folding (IDA)
Cellular Component
Manually curated	cytoplasm (IPI)

Regulatory Role

Regulatory modules	predicted: stressResponse (433)

Mutant phenotypes All PLP2 Phenotype evidence and references

Classical genetics
null	inviable
overexpression	pheromone sensitivity: normal
Large-scale survey
null	inviable viable
overexpression	vegetative growth: decreased rate vegetative growth: decreased
reduction of function	competitive fitness: decreased
repressible	chromosome/plasmid maintenance: decreased
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All PLP2 Interaction evidence and references

	34 total interaction(s) for 28 unique genes/features.
Physical Interactions	Affinity Capture-MS: 11 Affinity Capture-RNA: 2 Biochemical Activity: 2 Reconstituted Complex: 2 Two-hybrid: 4
Genetic Interactions	Dosage Growth Defect: 1 Dosage Rescue: 8 Synthetic Growth Defect: 2 Synthetic Lethality: 2
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All PLP2 Protein evidence and references

Localization	YeastRC \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| AspGD Homologs \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrXV:847129 to 846269 | |

Note: this feature is encoded on the Crick strand.

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..861	847129..846269	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000005807

SUMMARY PARAGRAPH for PLP2

PLP2 is one of two S. cerevisiae genes encoding proteins similar to mammalian phosducins (1). Phosducins inhibit the GTPase activity of several heterotrimeric G proteins, presumably as a consequence of binding to the beta-gamma subunits of the G proteins (5, 6).

Plp2p is essential for viability, but the other phosducin-related protein, Plp1p, is not (1). In yeast, a heterotrimeric G protein comprising Gpa1p, Ste4p, and Ste18p transduces the mating pheromone signal (reviewed in reference 7). Both Plp1p and Plp2p bind to the yeast G beta-gamma complex (Ste4p and Ste18p), suggesting that they may play a role in mating pheromone signal transduction (1). Overexpression of PLP1 or PLP2 reduces pheromone-responsive transcriptional activation, but has no effect on pheromone induced growth arrest (1). Deletion of STE7, which encodes a MAP kinase kinase involved in pheromone signal transduction downstream of the heterotrimeric G protein (7) does not rescue the plp2 null mutant, suggesting that Plp2p may have an essential role in a process other than mating (1).

Last updated: 2000-04-13

References cited on this page View Complete Literature Guide for PLP2

1)	Flanary PL, et al. (2000) Functional analysis of Plp1 and Plp2, two homologues of phosducin in yeast. J Biol Chem 275(24):18462-9
2)	Lopez P, et al. (2003) A novel germ line-specific gene of the phosducin-like protein (PhLP) family. A meiotic function conserved from yeast to mice. J Biol Chem 278(3):1751-7
3)	Dekker C, et al. (2008) The interaction network of the chaperonin CCT. EMBO J 27(13):1827-39
4)	McCormack EA, et al. (2009) Yeast phosducin-like protein 2 acts as a stimulatory co-factor for the folding of actin by the chaperonin CCT via a ternary complex. J Mol Biol 391(1):192-206
5)	Bauer PH, et al. (1992) Phosducin is a protein kinase A-regulated G-protein regulator. Nature 358(6381):73-6
6)	Lee RH, et al. (1992) Regulation of retinal cGMP cascade by phosducin in bovine rod photoreceptor cells. Interaction of phosducin and transducin. J Biol Chem 267(35):25104-12
7)	Sprague GF Jr and Thorner JW (1992) "Pheromone response and signal transduction during the mating process of Saccharomyces cerevisiae." Pp. 657-744 in The Molecular and Cellular Biology of the Yeast Saccharomyces: Gene Expression, edited by Jones EW, Pringle JR and Broach JR. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press