PLB1/YMR008C Summary Help

Standard Name PLB1 1
Systematic Name YMR008C
Feature Type ORF, Verified
Description Phospholipase B (lysophospholipase) involved in lipid metabolism; required for efficient acyl chain remodeling of newly synthesized phosphatidylethanolamine-derived phosphatidylcholine; required for deacylation of phosphatidylcholine and phosphatidylethanolamine but not phosphatidylinositol; PLB1 has a paralog, PLB3, that arose from the whole genome duplication (1, 2, 3, 4)
Name Description PhosphoLipase B 1
Chromosomal Location
ChrXIII:282584 to 280590 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All PLB1 GO evidence and references
  View Computational GO annotations for PLB1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 19 genes
Resources
Classical genetics
null
overexpression
Large-scale survey
null
overexpression
Resources
22 total interaction(s) for 21 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 2
  • Affinity Capture-RNA: 1
  • Protein-RNA: 1
  • Two-hybrid: 1

Genetic Interactions
  • Dosage Rescue: 4
  • Negative Genetic: 7
  • Phenotypic Enhancement: 2
  • Positive Genetic: 2
  • Synthetic Growth Defect: 1
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 664
Molecular Weight (Da) 71,667
Isoelectric Point (pI) 4.36
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIII:282584 to 280590 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 1996-07-31 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1995 282584..280590 1996-07-31 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004610
References cited on this page View Complete Literature Guide for PLB1
1) Lee KS, et al.  (1994) The Saccharomyces cerevisiae PLB1 gene encodes a protein required for lysophospholipase and phospholipase B activity. J Biol Chem 269(31):19725-30
2) Merkel O, et al.  (2005) Regulation of activity in vitro and in vivo of three phospholipases B from Saccharomyces cerevisiae. Biochem J 387(Pt 2):489-96
3) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
4) De Smet CH, et al.  (2013) Yeast cells accumulate excess endogenous palmitate in phosphatidylcholine by acyl chain remodeling involving the phospholipase B Plb1p. Biochim Biophys Acta 1831(6):1167-76