PKH1/YDR490C Summary

PKH1/YDR490C Summary

Standard Name	PKH1 1
Systematic Name	YDR490C
Feature Type	ORF, Verified
Description	Serine/threonine protein kinase; involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; redundant with Pkh2p; PKH1 has a paralog, PKH2, that arose from the whole genome duplication (1, 2, 3, 4, 5)
Name Description	Pkb-activating Kinase Homolog 1

Chromosomal Location	ChrIV:1434268 to 1431968 \| ORF Map \| GBrowse
	Note: this feature is encoded on the Crick strand.

Gene Ontology Annotations All PKH1 GO evidence and references

	View Computational GO annotations for PKH1
Molecular Function
Manually curated	protein kinase activity (IDA)
Biological Process
Manually curated	cytoplasmic mRNA processing body assembly (IGI) endocytosis (IMP) MAPK cascade involved in cell wall biogenesis (IGI, IMP) protein phosphorylation (IDA) regulation of nuclear-transcribed mRNA poly(A) tail shortening (IGI)
Cellular Component
Manually curated	cell cortex (IDA) cytosol (IDA) eisosome (IDA)

Mutant phenotypes All PKH1 Phenotype evidence and references

Classical genetics
null	chronological lifespan: increased
Large-scale survey
null	competitive fitness: decreased heat sensitivity: increased resistance to fluconazole: decreased resistance to caffeine: decreased resistance to cycloheximide: decreased resistance to bleomycin: decreased resistance to 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid: decreased resistance to 1,4-dithiothreitol and tacrolimus (anhydrous): increased resistance to tacrolimus (anhydrous) and tunicamycin: increased resistance to amitrole: decreased resistance to hydroxyurea: decreased resistance to methyl methanesulfonate: decreased resistance to tunicamycin: decreased resistance to L-canavanine: increased vacuolar morphology: abnormal viable
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All PKH1 Interaction evidence and references

	152 total interaction(s) for 112 unique genes/features.
Physical Interactions	Affinity Capture-MS: 15 Affinity Capture-Western: 4 Biochemical Activity: 12 PCA: 2 Reconstituted Complex: 10 Two-hybrid: 5
Genetic Interactions	Dosage Growth Defect: 7 Dosage Rescue: 1 Negative Genetic: 60 Phenotypic Enhancement: 1 Positive Genetic: 11 Synthetic Growth Defect: 19 Synthetic Lethality: 4 Synthetic Rescue: 1
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All PKH1 Protein evidence and references

Localization	YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrIV:1434268 to 1431968 | |

Note: this feature is encoded on the Crick strand.

SGD ORF map

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..2301	1434268..1431968	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| E.C. \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000002898

References cited on this page View Complete Literature Guide for PKH1

1)	Casamayor A, et al. (1999) Functional counterparts of mammalian protein kinases PDK1 and SGK in budding yeast. Curr Biol 9(4):186-97
2)	Sun Y, et al. (2000) Sli2 (Ypk1), a homologue of mammalian protein kinase SGK, is a downstream kinase in the sphingolipid-mediated signaling pathway of yeast. Mol Cell Biol 20(12):4411-9
3)	Friant S, et al. (2001) Sphingoid base signaling via Pkh kinases is required for endocytosis in yeast. EMBO J 20(23):6783-92
4)	Roelants FM, et al. (2002) Pkh1 and Pkh2 differentially phosphorylate and activate Ypk1 and Ykr2 and define protein kinase modules required for maintenance of cell wall integrity. Mol Biol Cell 13(9):3005-28
5)	Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61