PIR3/YKL163W Summary Help

Standard Name PIR3 1
Systematic Name YKL163W
Alias CCW8 2
Feature Type ORF, Verified
Description O-glycosylated covalently-bound cell wall protein; required for cell wall stability; expression is cell cycle regulated, peaking in M/G1 and also subject to regulation by the cell integrity pathway; coding sequence contains length polymorphisms in different strains; PIR3 has a paralog, HSP150, that arose from the whole genome duplication (2, 3, 4, 5, 6, 7, 8, 9)
Name Description Protein containing Internal Repeats 1
Chromosomal Location
ChrXI:144401 to 145378 | ORF Map | GBrowse
Gene Ontology Annotations All PIR3 GO evidence and references
  View Computational GO annotations for PIR3
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 11 genes
Classical genetics
Large-scale survey
9 total interaction(s) for 8 unique genes/features.
Physical Interactions
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 1
  • Protein-RNA: 1

Genetic Interactions
  • Negative Genetic: 2
  • Synthetic Growth Defect: 1
  • Synthetic Lethality: 1
  • Synthetic Rescue: 1

Expression Summary
Length (a.a.) 325
Molecular Weight (Da) 33,004
Isoelectric Point (pI) 5.32
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXI:144401 to 145378 | ORF Map | GBrowse
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..978 144401..145378 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001646
References cited on this page View Complete Literature Guide for PIR3
1) Toh-e A, et al.  (1993) Three yeast genes, PIR1, PIR2 and PIR3, containing internal tandem repeats, are related to each other, and PIR1 and PIR2 are required for tolerance to heat shock. Yeast 9(5):481-94
2) Mrsa V, et al.  (1997) Specific labelling of cell wall proteins by biotinylation. Identification of four covalently linked O-mannosylated proteins of Saccharomyces cerevisiae. Yeast 13(12):1145-54
3) Mrsa V and Tanner W  (1999) Role of NaOH-extractable cell wall proteins Ccw5p, Ccw6p, Ccw7p and Ccw8p (members of the Pir protein family) in stability of the Saccharomyces cerevisiae cell wall. Yeast 15(10A):813-20
4) Jung US and Levin DE  (1999) Genome-wide analysis of gene expression regulated by the yeast cell wall integrity signalling pathway. Mol Microbiol 34(5):1049-57
5) Yun DJ, et al.  (1997) Stress proteins on the yeast cell surface determine resistance to osmotin, a plant antifungal protein. Proc Natl Acad Sci U S A 94(13):7082-7
6) Doolin MT, et al.  (2001) Overlapping and distinct roles of the duplicated yeast transcription factors Ace2p and Swi5p. Mol Microbiol 40(2):422-32
7) Porter SE, et al.  (2002) The yeast pafl-rNA polymerase II complex is required for full expression of a subset of cell cycle-regulated genes. Eukaryot Cell 1(5):830-42
8) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
9) Carvalho-Netto OV, et al.  (2013) A simple and effective set of PCR-based molecular markers for the monitoring of the Saccharomyces cerevisiae cell population during bioethanol fermentation. J Biotechnol 168(4):701-9