PIF1/YML061C Summary Help

Standard Name PIF1 1, 2
Systematic Name YML061C
Alias TST1
Feature Type ORF, Verified
Description DNA helicase, potent G-quadruplex DNA binder/unwinder; promotes DNA synthesis during break-induced replication (BIR); important for crossover recombination; translated from different start sites for two forms localized to either mitochondria or nucleus; nuclear form is catalytic inhibitor of telomerase; mitochondrial form involved in mitochondrial DNA repair and recombination; mutations affect Zn, Fe homeostasis; regulated by Rad53p-dependent phosphorylation in rho0 cells (1, 3, 4, 5, 6, 7, 8, 9, 10)
Name Description Petite Integration Frequency 2
Chromosomal Location
ChrXIII:151532 to 148953 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All PIF1 GO evidence and references
  View Computational GO annotations for PIF1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 3 genes
Classical genetics
reduction of function
Large-scale survey
169 total interaction(s) for 105 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 6
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 3
  • Reconstituted Complex: 3

Genetic Interactions
  • Dosage Growth Defect: 40
  • Dosage Lethality: 5
  • Dosage Rescue: 4
  • Phenotypic Enhancement: 40
  • Phenotypic Suppression: 22
  • Positive Genetic: 1
  • Synthetic Growth Defect: 16
  • Synthetic Lethality: 5
  • Synthetic Rescue: 23

Expression Summary
Length (a.a.) 859
Molecular Weight (Da) 97,681
Isoelectric Point (pI) 9.72
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXIII:151532 to 148953 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 1996-07-31 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..2580 151532..148953 1996-07-31 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004526
References cited on this page View Complete Literature Guide for PIF1
1) Foury F and Lahaye A  (1987) Cloning and sequencing of the PIF gene involved in repair and recombination of yeast mitochondrial DNA. EMBO J 6(5):1441-9
2) Foury F and Kolodynski J  (1983) pif mutation blocks recombination between mitochondrial rho+ and rho- genomes having tandemly arrayed repeat units in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 80(17):5345-9
3) Ivessa AS, et al.  (2000) The Saccharomyces Pif1p DNA helicase and the highly related Rrm3p have opposite effects on replication fork progression in ribosomal DNA. Cell 100(4):479-89
4) Schulz VP and Zakian VA  (1994) The saccharomyces PIF1 DNA helicase inhibits telomere elongation and de novo telomere formation. Cell 76(1):145-55
5) Zhou J, et al.  (2000) Pif1p helicase, a catalytic inhibitor of telomerase in yeast. Science 289(5480):771-4
6) Vega LR, et al.  (2007) Sensitivity of yeast strains with long g-tails to levels of telomere-bound telomerase. PLoS Genet 3(6):e105
7) Guirola M, et al.  (2010) Lack of DNA helicase Pif1 disrupts zinc and iron homoeostasis in yeast. Biochem J 432(3):595-605
8) Crider DG, et al.  (2012) Rad53 is essential for a mitochondrial DNA inheritance checkpoint regulating G1 to S progression. J Cell Biol 198(5):793-8
9) Paeschke K, et al.  (2013) Pif1 family helicases suppress genome instability at G-quadruplex motifs. Nature 497(7450):458-62
10) Wilson MA, et al.  (2013) Pif1 helicase and Pold promote recombination-coupled DNA synthesis via bubble migration. Nature 502(7471):393-6