PHS1/YJL097W Summary Help

Standard Name PHS1 1
Systematic Name YJL097W
Feature Type ORF, Verified
Description Essential 3-hydroxyacyl-CoA dehydratase of the ER membrane; involved in elongation of very long-chain fatty acids; evolutionarily conserved, similar to mammalian PTPLA and PTPLB; involved in sphingolipid biosynthesis and protein trafficking (1, 2, 3)
Name Description PTPLA Homolog involved in Sphingolipid biosynthesis 1 1
Chromosomal Location
ChrX:245591 to 246244 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All PHS1 GO evidence and references
  View Computational GO annotations for PHS1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 2 genes
Resources
Pathways
Classical genetics
repressible
unspecified
Large-scale survey
conditional
null
overexpression
reduction of function
repressible
Resources
36 total interaction(s) for 27 unique genes/features.
Physical Interactions
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 3
  • Two-hybrid: 8

Genetic Interactions
  • Negative Genetic: 20
  • Positive Genetic: 2
  • Synthetic Growth Defect: 1
  • Synthetic Lethality: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 217
Molecular Weight (Da) 24,510
Isoelectric Point (pI) 10.84
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrX:245591 to 246244 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..654 245591..246244 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003633
References cited on this page View Complete Literature Guide for PHS1
1) Schuldiner M, et al.  (2005) Exploration of the function and organization of the yeast early secretory pathway through an epistatic miniarray profile. Cell 123(3):507-19
2) Yu L, et al.  (2006) A survey of essential gene function in the yeast cell division cycle. Mol Biol Cell 17(11):4736-47
3) Kihara A, et al.  (2008) Membrane Topology and Essential Amino Acid Residues of Phs1, a 3-Hydroxyacyl-CoA Dehydratase Involved in Very Long-chain Fatty Acid Elongation. J Biol Chem 283(17):11199-209