Standard Name	PHO84
Systematic Name	YML123C
Feature Type	ORF, Verified
Description	High-affinity inorganic phosphate (Pi) transporter and low-affinity manganese transporter; regulated by Pho4p and Spt7p; mutation confers resistance to arsenate; exit from the ER during maturation requires Pho86p; cells overexpressing Pho84p accumulate heavy metals but do not develop symptoms of metal toxicity (1, 2, 3, 4, 5) Also known as: phoT 6
Name Description	PHOsphate metabolism

Chromosomal Location	ChrXIII:25800 to 24037 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.
	Genetic position: -100.5 cM

	View Computational GO annotations for PHO84
Molecular Function
Manually curated	inorganic phosphate transmembrane transporter activity (IDA, IMP, ISS) manganese ion transmembrane transporter activity (IMP)
Biological Process
Manually curated	manganese ion transport (IMP) phosphate ion transport (IDA, IMP, ISS) polyphosphate metabolic process (IMP)
Cellular Component
Manually curated	integral to plasma membrane (IDA, ISS)
High-throughput	integral to membrane (ISM)

Classical genetics
conditional	acid phosphatase activity: increased
null	copper(2+) accumulation: decreased manganese(2+) accumulation: decreased cobalt(2+) accumulation: decreased N-ribosylnicotinamide accumulation: increased zinc(2+) accumulation: decreased polyphosphate accumulation: decreased N-ribosylnicotinamide excretion: increased metal resistance: increased nutrient uptake: absent resistance to sirolimus: increased resistance to quinine: increased resistance to arsenate(3-): decreased resistance to disodium selenite: decreased small molecule transport: decreased rate vegetative growth: absent
reduction of function	acid phosphatase activity: increased
Large-scale survey
null	competitive fitness: decreased fermentative growth: decreased rate haploinsufficient resistance to bleomycin: increased resistance to fluconazole: increased resistance to methyl methanesulfonate: increased resistance to sirolimus: decreased resistance to 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid: decreased resistance to cycloheximide: decreased resistance to fenpropimorph: decreased resistance to wortmannin: normal resistance to tunicamycin: increased resistance to calcium dichloride: decreased utilization of phosphorus source: decreased viable
overexpression	vegetative growth: decreased rate
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	164 total interaction(s) for 133 unique genes/features.
Physical Interactions	Affinity Capture-MS: 15 Affinity Capture-RNA: 4 Affinity Capture-Western: 1 PCA: 18 Protein-RNA: 1
Genetic Interactions	Dosage Rescue: 3 Negative Genetic: 70 Phenotypic Enhancement: 8 Phenotypic Suppression: 13 Positive Genetic: 14 Synthetic Growth Defect: 6 Synthetic Lethality: 6 Synthetic Rescue: 5
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder | YeastRC Mass Spec

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | AspGD Homologs | CGD Homologs | CandidaDB Homologs | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrXIII:25800 to 24037 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.
	Genetic position: -100.5 cM
Last Update	Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..1764 25800..24037 2011-02-03 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI1631 S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FL100 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain M22 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | TCDB | UniProtKB

Primary SGDID	S000004592