PGM1/YKL127W Summary Help

Standard Name PGM1 1
Systematic Name YKL127W
Feature Type ORF, Verified
Description Phosphoglucomutase, minor isoform; catalyzes the conversion from glucose-1-phosphate to glucose-6-phosphate, which is a key step in hexose metabolism; PGM1 has a paralog, PGM2, that arose from the whole genome duplication (1, 2 and see Summary Paragraph)
Name Description PhosphoGlucoMutase 3
Chromosomal Location
ChrXI:203541 to 205253 | ORF Map | GBrowse
Gbrowse
Genetic position: -108.4 cM
Gene Ontology Annotations All PGM1 GO evidence and references
  View Computational GO annotations for PGM1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 10 genes
Resources
Pathways
Classical genetics
null
Large-scale survey
null
overexpression
Resources
98 total interaction(s) for 80 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 8
  • Affinity Capture-RNA: 3
  • Biochemical Activity: 4
  • Co-purification: 1
  • Reconstituted Complex: 1

Genetic Interactions
  • Dosage Rescue: 7
  • Negative Genetic: 62
  • Phenotypic Enhancement: 1
  • Phenotypic Suppression: 1
  • Positive Genetic: 5
  • Synthetic Growth Defect: 1
  • Synthetic Lethality: 2
  • Synthetic Rescue: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 570
Molecular Weight (Da) 63,111
Isoelectric Point (pI) 7.23
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXI:203541 to 205253 | ORF Map | GBrowse
SGD ORF map
Genetic position: -108.4 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1713 203541..205253 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001610
SUMMARY PARAGRAPH for PGM1

Phosphoglucomutase (EC:5.4.2.2) catalyzes the interconversion of glucose-6-phosphate and glucose-1-phosphate and is important for carbohydrate metabolism in a variety of organisms, ranging from bacteria to humans (4, 5, 1, 6). The direction of the interconversion is determined by the availability of substrate carbon sources (5). Saccharomyces cerevisiae contains a major phosphoglucomutase isoform, Pgm2p, and a minor phosphoglucomutase isoform, Pgm1p. Pgm2p and Pgm1p functions are involved in glycolysis, the pentose phosphate shunt, and the metabolism of glycogen, trehalose, and galactose. Phosphoglucomutase is also required for the synthesis of N-linked glycoproteins, extracellular glycans, and UDP-glucose (4, 5, 6, 7). Phosphoglucomutase also indirectly effects calcium uptake and homeostasis because glucose-1-phosphate and glucose-6-phosphate effect cation uptake (8, 9, 5).

PGM1 is constitutively expressed (5), and pgm1 null mutants are viable (10). pgm1 pgm2 double null mutants are also viable, but cannot use galactose as a sole carbon source, and accumulate lower levels of glycogen and trehalose than wild type (1).

Last updated: 2005-09-08 Contact SGD

References cited on this page View Complete Literature Guide for PGM1
1) Boles E, et al.  (1994) A family of hexosephosphate mutases in Saccharomyces cerevisiae. Eur J Biochem 220(1):83-96
2) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
3) Bevan P and Douglas HC  (1969) Genetic control of phosphoglucomutase variants in Saccharomyces cerevisiae. J Bacteriol 98(2):532-5
4) Dey NB, et al.  (1994) The glycosylation of phosphoglucomutase is modulated by carbon source and heat shock in Saccharomyces cerevisiae. J Biol Chem 269(43):27143-8
5) Fu L, et al.  (2000) Loss of the major isoform of phosphoglucomutase results in altered calcium homeostasis in Saccharomyces cerevisiae. J Biol Chem 275(8):5431-40
6) Aiello DP, et al.  (2002) Intracellular glucose 1-phosphate and glucose 6-phosphate levels modulate Ca2+ homeostasis in Saccharomyces cerevisiae. J Biol Chem 277(48):45751-8
7) Daran JM, et al.  (1997) Physiological and morphological effects of genetic alterations leading to a reduced synthesis of UDP-glucose in Saccharomyces cerevisiae. FEMS Microbiol Lett 153(1):89-96
8) Aiello DP, et al.  (2004) The Ca2+ homeostasis defects in a pgm2Delta strain of Saccharomyces cerevisiae are caused by excessive vacuolar Ca2+ uptake mediated by the Ca2+-ATPase Pmc1p. J Biol Chem 279(37):38495-502
9) Mulet JM, et al.  (2004) The trehalose pathway and intracellular glucose phosphates as modulators of potassium transport and general cation homeostasis in yeast. Yeast 21(7):569-82
10) Csutora P, et al.  (2005) Inhibition of phosphoglucomutase activity by lithium alters cellular calcium homeostasis and signaling in Saccharomyces cerevisiae. Am J Physiol Cell Physiol 289(1):C58-67