PGI1/YBR196C Summary Help

Standard Name PGI1 1
Systematic Name YBR196C
Alias CDC30
Feature Type ORF, Verified
Description Glycolytic enzyme phosphoglucose isomerase; catalyzes the interconversion of glucose-6-phosphate and fructose-6-phosphate; required for cell cycle progression and completion of the gluconeogenic events of sporulation (2, 3, 4, 5 and see Summary Paragraph)
Name Description PhosphoGlucoIsomerase 6
Chromosomal Location
ChrII:613900 to 612236 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Genetic position: 89 cM
Gene Ontology Annotations All PGI1 GO evidence and references
  View Computational GO annotations for PGI1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 12 genes
Resources
Pathways
Classical genetics
conditional
Large-scale survey
null
reduction of function
repressible
unspecified
Resources
64 total interaction(s) for 57 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 39
  • Affinity Capture-RNA: 6
  • Affinity Capture-Western: 1
  • Biochemical Activity: 1
  • PCA: 1
  • Two-hybrid: 1

Genetic Interactions
  • Dosage Rescue: 1
  • Negative Genetic: 8
  • Phenotypic Enhancement: 2
  • Phenotypic Suppression: 1
  • Positive Genetic: 2
  • Synthetic Lethality: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 554
Molecular Weight (Da) 61,299
Isoelectric Point (pI) 6.43
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrII:613900 to 612236 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Genetic position: 89 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1997-01-28
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1665 613900..612236 2011-02-03 1997-01-28
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000400
SUMMARY PARAGRAPH for PGI1

Glycolysis is the lysis, or splitting, of one molecule of glucose into two molecules of pyruvate, producing a net gain of two ATP molecules. Pyruvate can then be used in anaerobic (fermentation) or aerobic (respiration) metabolism. The glycolysis pathway and the genes involved are illustrated here.

PGI1 encodes phosphoglucose isomerase, a tetrameric enzyme that catalyzes the conversion of glucose-6-phosphate to fructose-6-phosphate during glycolysis and the reverse reaction during gluconeogenesis (5, 1, 6)

Gluconeogenesis is the process whereby glucose is synthesized from non-carbohydrate precursors, which enables yeast cells to grow on non-sugar carbon sources like ethanol, glycerol, or peptone. The reactions of gluconeogenesis, shown here, mediate conversion of pyruvate to glucose, which is the opposite of glycolysis, the formation of pyruvate from glucose. While these two pathways have several reactions in common, they are not the exact reverse of each other. As the glycolytic enzymes phosphofructokinase (Pfk1p, Pfk2p) and pyruvate kinase (Cdc19p) only function in the forward direction, the gluconeogenesis pathway replaces those steps with the enzymes pyruvate carboxylase (Pyc1p, Pyc2p) and phosphoenolpyruvate carboxykinase (Pck1p)-generating oxaloacetate as an intermediate from pyruvate to phosphoenolpyruvate-and also the enzyme fructose-1,6-bisphosphatase (Fbp1p) (reviewed in 7). Overall, the gluconeogenic reactions convert two molecules of pyruvate to a molecule of glucose, with the expenditure of six high-energy phosphate bonds, four from ATP and two from GTP.

Cells lacking Pgi1p are unable to grow on fructose or glucose as a sole carbon source, and both growth and sporulation are glucose dependent (2). Because fructose 6-phosphate (F6-P) is also a precursor of the cell wall components chitin and mannoprotein, temperature-sensitive (cdc30) mutations in phosphoglucose isomerase cause cell-cycle arrest probably due to an inability to synthesize components needed for cytokinesis and cell separation (8). Transcription of PGI1 does not appear to be regulated by glycolytic or gluconeogenic growth, or between exponential and stationary phases (9).

Last updated: 2005-07-22 Contact SGD

References cited on this page View Complete Literature Guide for PGI1
1) Maitra PK and Lobo Z  (1977) Genetic studies with a phosphoglucose isomerase mutant of Saccharomyces cerevisiae. Mol Gen Genet 156(1):55-60
2) Aguilera A  (1986) Deletion of the phosphoglucose isomerase structural gene makes growth and sporulation glucose dependent in Saccharomyces cerevisiae. Mol Gen Genet 204(2):310-6
3) Dickinson JR and Williams AS  (1987) The cdc30 mutation in Saccharomyces cerevisiae results in a temperature-sensitive isoenzyme of phosphoglucose isomerase. J Gen Microbiol 133(1):135-40
4) Dickinson JR, et al.  (1988) The cdc30 mutation in Saccharomyces cerevisiae affects phosphoglucose isomerase, the cell cycle and sporulation. J Gen Microbiol 134(9):2475-80
5) Aguilera A and Zimmermann FK  (1986) Isolation and molecular analysis of the phosphoglucose isomerase structural gene of Saccharomyces cerevisiae. Mol Gen Genet 202(1):83-9
6) Lowe SL and Reithel FJ  (1975) The subunit structure of phosphoglucose isomerase from bakers' yeast. J Biol Chem 250(1):94-9
7) Klein CJ, et al.  (1998) Glucose control in Saccharomyces cerevisiae: the role of Mig1 in metabolic functions. Microbiology 144 ( Pt 1)():13-24
8) Dickinson JR  (1991) Biochemical and genetic studies on the function of, and relationship between, the PGI1- and CDC30-encoded phosphoglucose isomerases in Saccharomyces cerevisiae. J Gen Microbiol 137(4):765-70
9) Green JB, et al.  (1988) The structure and regulation of phosphoglucose isomerase in Saccharomyces cerevisiae. Mol Gen Genet 215(1):100-6