PFA4/YOL003C Summary Help

Standard Name PFA4 1, 2
Systematic Name YOL003C
Feature Type ORF, Verified
Description Palmitoyltransferase with autoacylation activity; required for palmitoylation of amino acid permeases containing a C-terminal Phe-Trp-Cys site; required for modification of Chs3p; member of the DHHC family of putative palmitoyltransferases (2, 3, 4, 5)
Name Description Protein Fatty Acyltransferase 1
Chromosomal Location
ChrXV:322994 to 321858 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All PFA4 GO evidence and references
  View Computational GO annotations for PFA4
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 3 genes
Resources
Classical genetics
null
overexpression
unspecified
Large-scale survey
null
overexpression
Resources
67 total interaction(s) for 58 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 1
  • Affinity Capture-Western: 1
  • Biochemical Activity: 1
  • PCA: 10
  • Two-hybrid: 3

Genetic Interactions
  • Negative Genetic: 38
  • Phenotypic Enhancement: 3
  • Phenotypic Suppression: 1
  • Synthetic Growth Defect: 5
  • Synthetic Lethality: 4

Resources
Expression Summary
histogram
Resources
Length (a.a.) 378
Molecular Weight (Da) 44,488
Isoelectric Point (pI) 8.2
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXV:322994 to 321858 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1137 322994..321858 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005363
References cited on this page View Complete Literature Guide for PFA4
1) Smotrys J, et al.  (2005) personal communication
2) Lam KK, et al.  (2006) Palmitoylation by the DHHC protein Pfa4 regulates the ER exit of Chs3. J Cell Biol 174(1):19-25
3) Valdez-Taubas J and Pelham H  (2005) Swf1-dependent palmitoylation of the SNARE Tlg1 prevents its ubiquitination and degradation. EMBO J 24(14):2524-32
4) Smotrys JE, et al.  (2005) The vacuolar DHHC-CRD protein Pfa3p is a protein acyltransferase for Vac8p. J Cell Biol 170(7):1091-9
5) Roth AF, et al.  (2006) Global analysis of protein palmitoylation in yeast. Cell 125(5):1003-13