PEX7/YDR142C Summary Help

Standard Name PEX7 1
Systematic Name YDR142C
Alias PAS7 2 , PEB1 3
Feature Type ORF, Verified
Description Peroxisomal signal receptor for peroxisomal matrix proteins; recognizes the N-terminal nonapeptide signal (PTS2); WD repeat protein; defects in human homolog cause lethal rhizomelic chondrodysplasia punctata (RCDP) (3, 4, 5 and see Summary Paragraph)
Name Description PEroXin 1
Chromosomal Location
ChrIV:741600 to 740473 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All PEX7 GO evidence and references
  View Computational GO annotations for PEX7
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 1 genes
Resources
Classical genetics
reduction of function
Large-scale survey
null
overexpression
Resources
66 total interaction(s) for 28 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 14
  • Affinity Capture-Western: 15
  • Co-crystal Structure: 1
  • Co-purification: 1
  • PCA: 1
  • Reconstituted Complex: 6
  • Two-hybrid: 25

Genetic Interactions
  • Negative Genetic: 2
  • Positive Genetic: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 375
Molecular Weight (Da) 42,323
Isoelectric Point (pI) 8.34
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:741600 to 740473 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1128 741600..740473 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002549
SUMMARY PARAGRAPH for PEX7

The biogenesis of peroxisomes requires a group of protein factors referred to as peroxins which are encoded by the PEX genes. Peroxisomal proteins are synthesized on free polyribosomes and imported posttranslationally. The transport of peroxisomal matrix proteins from the cytoplasm to the peroxisome is mediated by two peroxisome-targeting signal sequences (PTS1 and PTS2). Peroxisomal membrane proteins (PMPs) are imported independently of the matrix proteins by a distinct mechanism mediated by the membrane PTS signal (mPTS) (6, 7, 8, 9 and references therein).

Pex7p, Pex18p, and Pex21p are involved in peroxisome biogenesis and the import of peroxisomal matrix proteins that contain the peroxisomal targeting sequence PTS2 (8, 10). The PTS2 is located within the first 20 amino acids and has the consensus sequence H/R-L-X5-H-L (11, 12). Pex7p binds PTS2-containing proteins in the cytoplasm and delivers them to the peroxisomal membrane where Pex18p and Pex21p contribute to their interaction with the peroxisomal import machinery (5, 13, 14).

Two subcomplexes of the peroxisomal import machinery have been defined: the docking subcomplex comprises Pex14p, Pex17p, and Pex13p, while the translocation subcomplex contains Pex2p, Pex10p, and Pex12p. The proteins of the translocation complex expose their RING finger domains to the outer face of the peroxisomal membrane, and act downstream of Pex14p, Pex17p, and Pex13p during the peroxisomal protein import process (15). Association of both subcomplexes into a larger import complex requires Pex8p, an intraperoxisomal protein. Pex8p organizes the formation of the larger import complex from the trans side of the peroxisomal membrane and thus might enable functional communication between both sides of the membrane (15).

Localization studies suggest that Pex7p shuttles between the cytoplasm and the peroxisomal matrix, as Pex7p with an amino-terminal tag localized to the cytoplasm while Pex7p with a carboxy-terminal tag primarily localized to the peroxisome (2, 3, 16). Although peroxisome biogenesis can be induced by growing S. cerevisiae on oleic acid, PEX7 expression was not induced in the presence of oleic acid (8, 17).

Pex7p is a member of the family of proteins that contain WD repeats (2, 3). Orthologs of PEX7 have been identified in other fungi, plants, and humans but not in nematodes (18, 19, 20, 21, 22). The human PEX7 is responsible for the rare developmental disorder rhizomelic chondrodysplasia punctata (RCDP) (reviewed in 23).

Last updated: 2007-06-28 Contact SGD

References cited on this page View Complete Literature Guide for PEX7
1) Distel B, et al.  (1996) A unified nomenclature for peroxisome biogenesis factors. J Cell Biol 135(1):1-3
2) Marzioch M, et al.  (1994) PAS7 encodes a novel yeast member of the WD-40 protein family essential for import of 3-oxoacyl-CoA thiolase, a PTS2-containing protein, into peroxisomes. EMBO J 13(20):4908-18
3) Zhang JW and Lazarow PB  (1995) PEB1 (PAS7) in Saccharomyces cerevisiae encodes a hydrophilic, intra-peroxisomal protein that is a member of the WD repeat family and is essential for the import of thiolase into peroxisomes. J Cell Biol 129(1):65-80
4) Erdmann R and Blobel G  (1996) Identification of Pex13p a peroxisomal membrane receptor for the PTS1 recognition factor. J Cell Biol 135(1):111-21
5) Purdue PE, et al.  (1998) Pex18p and Pex21p, a novel pair of related peroxins essential for peroxisomal targeting by the PTS2 pathway. J Cell Biol 143(7):1859-69
6) Hettema EH, et al.  (2000) Saccharomyces cerevisiae pex3p and pex19p are required for proper localization and stability of peroxisomal membrane proteins. EMBO J 19(2):223-33
7) Sacksteder KA and Gould SJ  (2000) The genetics of peroxisome biogenesis. Annu Rev Genet 34:623-652
8) Purdue PE and Lazarow PB  (2001) Peroxisome biogenesis. Annu Rev Cell Dev Biol 17:701-52
9) Fujiki Y, et al.  (2006) Import of peroxisomal membrane proteins: The interplay of Pex3p- and Pex19p-mediated interactions. Biochim Biophys Acta 1763(12):1639-46
10) Brown LA and Baker A  (2003) Peroxisome biogenesis and the role of protein import. J Cell Mol Med 7(4):388-400
11) Glover JR, et al.  (1994) Mutagenesis of the amino targeting signal of Saccharomyces cerevisiae 3-ketoacyl-CoA thiolase reveals conserved amino acids required for import into peroxisomes in vivo. J Biol Chem 269(10):7558-63
12) Zhang JW and Lazarow PB  (1996) Peb1p (Pas7p) is an intraperoxisomal receptor for the NH2-terminal, type 2, peroxisomal targeting sequence of thiolase: Peb1p itself is targeted to peroxisomes by an NH2-terminal peptide. J Cell Biol 132(3):325-34
13) Stein K, et al.  (2002) Interactions of Pex7p and Pex18p/Pex21p with the peroxisomal docking machinery: implications for the first steps in PTS2 protein import. Mol Cell Biol 22(17):6056-69
14) Heiland I and Erdmann R  (2005) Biogenesis of peroxisomes. Topogenesis of the peroxisomal membrane and matrix proteins. FEBS J 272(10):2362-72
15) Agne B, et al.  (2003) Pex8p: an intraperoxisomal organizer of the peroxisomal import machinery. Mol Cell 11(3):635-46
16) Nair DM, et al.  (2004) Pex7p translocates in and out of peroxisomes in Saccharomyces cerevisiae. J Cell Biol 167(4):599-604
17) Rottensteiner H, et al.  (2003) Saccharomyces cerevisiae Pip2p-Oaf1p regulates PEX25 transcription through an adenine-less ORE. Eur J Biochem 270(9):2013-22
18) Kiel JA, et al.  (2006) PEX Genes in Fungal Genomes: Common, Rare or Redundant. Traffic 7(10):1291-303
19) Li DM, et al.  (2003) [Cloning and characterization of the PTS2 receptor gene (GhPex7) from cotton (Gossypium hirsutum L.)] Yi Chuan Xue Bao 30(9):823-9
20) Braverman N, et al.  (1997) Human PEX7 encodes the peroxisomal PTS2 receptor and is responsible for rhizomelic chondrodysplasia punctata. Nat Genet 15(4):369-76
21) Motley AM, et al.  (1997) Rhizomelic chondrodysplasia punctata is a peroxisomal protein targeting disease caused by a non-functional PTS2 receptor. Nat Genet 15(4):377-80
22) Purdue PE, et al.  (1997) Rhizomelic chondrodysplasia punctata is caused by deficiency of human PEX7, a homologue of the yeast PTS2 receptor. Nat Genet 15(4):381-4
23) Purdue PE, et al.  (1999) Rhizomelic chondrodysplasia punctata, a peroxisomal biogenesis disorder caused by defects in Pex7p, a peroxisomal protein import receptor: a minireview. Neurochem Res 24(4):581-6