PEX27/YOR193W Summary Help

Standard Name PEX27 1
Systematic Name YOR193W
Feature Type ORF, Verified
Description Peripheral peroxisomal membrane protein; involved in controlling peroxisome size and number, interacts with Pex25p; PEX27 has a paralog, PEX25, that arose from the whole genome duplication (1, 2, 3 and see Summary Paragraph)
Name Description PEroXisome related 2
Chromosomal Location
ChrXV:710446 to 711576 | ORF Map | GBrowse
Gene Ontology Annotations All PEX27 GO evidence and references
  View Computational GO annotations for PEX27
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 8 genes
Classical genetics
Large-scale survey
20 total interaction(s) for 14 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 1
  • Affinity Capture-RNA: 1
  • Biochemical Activity: 1
  • Protein-peptide: 1
  • Two-hybrid: 3

Genetic Interactions
  • Dosage Growth Defect: 2
  • Dosage Lethality: 1
  • Negative Genetic: 3
  • Phenotypic Enhancement: 1
  • Phenotypic Suppression: 1
  • Positive Genetic: 2
  • Synthetic Lethality: 2
  • Synthetic Rescue: 1

Expression Summary
Length (a.a.) 376
Molecular Weight (Da) 44,131
Isoelectric Point (pI) 10.49
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXV:710446 to 711576 | ORF Map | GBrowse
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1131 710446..711576 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005719

Pex11p, Pex25p, and Pex27p comprise a family of proteins whose members are required for peroxisome biogenesis and play a role in the regulation of peroxisome size and number (2). Pex25p and Pex27p are peroxisomal membrane proteins whose C-termini are similar to the entire Pex11p. Deletion of PEX25 or PEX27 results in enlarged peroxisomes. A partial growth defect on fatty acids of a pex25delta mutant is not exacerbated by the additional deletion of PEX27; however, when PEX11 is also deleted, growth is abolished on all fatty acids. Moreover, the triple mutant strain exhibits a severe peroxisomal protein import defect (2). Overexpression of PEX27 or PEX25 leads to peroxisome proliferation and the formation of small peroxisomes. Overexpression of PEX11 has been reported to produce this same small-peroxisome phenotype (1), and also a similar phenotype in which cells contain an increased number of normal-sized peroxisomes (4).

Last updated: 2005-03-17 Contact SGD

References cited on this page View Complete Literature Guide for PEX27
1) Tam YY, et al.  (2003) Pex11-related proteins in peroxisome dynamics: a role for the novel peroxin Pex27p in controlling peroxisome size and number in Saccharomyces cerevisiae. Mol Biol Cell 14(10):4089-102
2) Rottensteiner H, et al.  (2003) Conserved function of pex11p and the novel pex25p and pex27p in peroxisome biogenesis. Mol Biol Cell 14(10):4316-28
3) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
4) Marshall PA, et al.  (1995) Pmp27 promotes peroxisomal proliferation. J Cell Biol 129(2):345-55