PEX13/YLR191W Summary Help

Standard Name PEX13 1, 2
Systematic Name YLR191W
Alias PAS20 3
Feature Type ORF, Verified
Description Integral peroxisomal membrane protein required for translocation of peroxisomal matrix proteins, interacts with the PTS1 signal recognition factor Pex5p and the PTS2 signal recognition factor Pex7p, forms a complex with Pex14p and Pex17p (4, 5, 6, 7 and see Summary Paragraph)
Name Description PEroXin 2
Chromosomal Location
ChrXII:537272 to 538432 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All PEX13 GO evidence and references
  View Computational GO annotations for PEX13
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Mutant phenotypes All PEX13 Phenotype evidence and references
Classical genetics
null
reduction of function
Large-scale survey
null
overexpression
Resources
interactions All PEX13 Interaction evidence and references
185 total interaction(s) for 122 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 6
  • Affinity Capture-Western: 18
  • Co-crystal Structure: 2
  • Co-fractionation: 4
  • Co-localization: 1
  • Co-purification: 2
  • PCA: 19
  • Protein-peptide: 2
  • Reconstituted Complex: 5
  • Two-hybrid: 67
Genetic Interactions
  • Dosage Lethality: 2
  • Negative Genetic: 42
  • Positive Genetic: 4
  • Synthetic Growth Defect: 8
  • Synthetic Lethality: 2
  • Synthetic Rescue: 1
Resources
Expression Summary
histogram
Resources
Protein Information All PEX13 Protein evidence and references
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXII:537272 to 538432 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..1161 537272..538432 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004181
SUMMARY PARAGRAPH for PEX13

The biogenesis of peroxisomes requires a group of protein factors referred to as peroxins which are encoded by the PEX genes. Peroxisomal proteins are synthesized on free polyribosomes and imported posttranslationally. The transport of peroxisomal matrix proteins from the cytoplasm to the peroxisome is mediated by two peroxisome-targeting signal sequences (PTS1 and PTS2). Peroxisomal membrane proteins (PMPs) are imported independently of the matrix proteins by a distinct mechanism mediated by the membrane PTS signal (mPTS) (8, 9, 10, 11 and references therein).

Pex13p, Pex14p, and Pex17p form a docking complex that is required for PTS1 and PTS2 peroxisomal matrix proteins to associate with the peroxisomal membrane prior to import into the peroxisomal matrix (reviewed in 12, 13). Although Pex13p and Pex14p interact with both Pex5p (the receptor for PTS1) and Pex7p (the receptor for PTS2), Pex14p may be the initial docking site (14, 4, 15, 15). All members of the docking complex are localized to the cytosolic face of the peroxisomal membrane. Pex13 is an integral membrane protein with its amino and carboxy termini exposed to the cytoplasm, while Pex14 and Pex17 are peripheral membrane proteins (4, 14, 16). Therefore, interactions between the docking complex and the translocation complex comprised of Pex2p, Pex10p, and Pex12p may enable communication between both sides of the peroxisomal membrane (17).

Multiple interactions occur among the proteins of the docking complex. Pex14p interacts with Pex13p, an interaction that may be mediated by Pex5p (14, 18). Pex14p also interacts with Pex17p, even in the absence of Pex13p (16). Biochemical purification of the docking complex indicates that only 10% of Pex13p is located there, suggesting Pex13p may have additional functions (17, 19).

Of the three proteins, Pex13p and Pex14 are widely conserved but Pex17p has only been identified in ascomycetes such as S. cerevisiae and C. albicans (20). Mutations in the human Pex13 and Pex14 have been associated with the peroxisomal biogenesis disorders neonatal adrenoleukodystrophy and Zellweger syndrome (21, 22, and references therein).

Last updated: 2007-07-05

References cited on this page View Complete Literature Guide for PEX13
1) Erdmann R and Blobel G  (1996) Identification of Pex13p a peroxisomal membrane receptor for the PTS1 recognition factor. J Cell Biol 135(1):111-21
2) Distel B, et al.  (1996) A unified nomenclature for peroxisome biogenesis factors. J Cell Biol 135(1):1-3
3) Elgersma Y, et al.  (1993) An efficient positive selection procedure for the isolation of peroxisomal import and peroxisome assembly mutants of Saccharomyces cerevisiae. Genetics 135(3):731-40
4) Girzalsky W, et al.  (1999) Involvement of Pex13p in Pex14p localization and peroxisomal targeting signal 2-dependent protein import into peroxisomes. J Cell Biol 144(6):1151-62
5) Gould SJ, et al.  (1996) Pex13p is an SH3 protein of the peroxisome membrane and a docking factor for the predominantly cytoplasmic PTs1 receptor. J Cell Biol 135(1):85-95
6) Pires JR, et al.  (2003) The ScPex13p SH3 domain exposes two distinct binding sites for Pex5p and Pex14p. J Mol Biol 326(5):1427-35
7) Girzalsky W, et al.  (2006) Pex19p-dependent targeting of Pex17p, a peripheral component of the peroxisomal protein import machinery. J Biol Chem 281(28):19417-25
8) Hettema EH, et al.  (2000) Saccharomyces cerevisiae pex3p and pex19p are required for proper localization and stability of peroxisomal membrane proteins. EMBO J 19(2):223-33
9) Sacksteder KA and Gould SJ  (2000) The genetics of peroxisome biogenesis. Annu Rev Genet 34:623-652
10) Purdue PE and Lazarow PB  (2001) Peroxisome biogenesis. Annu Rev Cell Dev Biol 17:701-52
11) Fujiki Y, et al.  (2006) Import of peroxisomal membrane proteins: The interplay of Pex3p- and Pex19p-mediated interactions. Biochim Biophys Acta 1763(12):1639-46
12) Heiland I and Erdmann R  (2005) Biogenesis of peroxisomes. Topogenesis of the peroxisomal membrane and matrix proteins. FEBS J 272(10):2362-72
13) Brown LA and Baker A  (2003) Peroxisome biogenesis and the role of protein import. J Cell Mol Med 7(4):388-400
14) Albertini M, et al.  (1997) Pex14p, a peroxisomal membrane protein binding both receptors of the two PTS-dependent import pathways. Cell 89(1):83-92
15) Niederhoff K, et al.  (2005) Yeast Pex14p possesses two functionally distinct Pex5p and one Pex7p binding sites. J Biol Chem 280(42):35571-8
16) Huhse B, et al.  (1998) Pex17p of Saccharomyces cerevisiae is a novel peroxin and component of the peroxisomal protein translocation machinery. J Cell Biol 140(1):49-60
17) Agne B, et al.  (2003) Pex8p: an intraperoxisomal organizer of the peroxisomal import machinery. Mol Cell 11(3):635-46
18) Schell-Steven A, et al.  (2005) Identification of a novel, intraperoxisomal pex14-binding site in pex13: association of pex13 with the docking complex is essential for peroxisomal matrix protein import. Mol Cell Biol 25(8):3007-18
19) Williams C and Distel B  (2006) Pex13p: Docking or cargo handling protein? Biochim Biophys Acta 1763(12):1585-91
20) Kiel JA, et al.  (2006) PEX Genes in Fungal Genomes: Common, Rare or Redundant. Traffic 7(10):1291-303
21) Bottger G, et al.  (2000) Saccharomyces cerevisiae PTS1 receptor Pex5p interacts with the SH3 domain of the peroxisomal membrane protein Pex13p in an unconventional, non-PXXP-related manner. Mol Biol Cell 11(11):3963-76
22) Azevedo JE and Schliebs W  (2006) Pex14p, more than just a docking protein. Biochim Biophys Acta 1763(12):1574-84