PEX11/YOL147C Summary Help

Standard Name PEX11 1
Systematic Name YOL147C
Alias PMP24 2 , PMP27 3 , 4
Feature Type ORF, Verified
Description Peroxisomal protein required for medium-chain fatty acid oxidation; also required for peroxisome proliferation, possibly by inducing membrane curvature; localization regulated by phosphorylation; transcription regulated by Adr1p and Pip2p-Oaf1p (3, 5, 6, 7, 8 and see Summary Paragraph)
Name Description PEroXin 1
Chromosomal Location
ChrXV:48643 to 47933 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All PEX11 GO evidence and references
  View Computational GO annotations for PEX11
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 79 genes
Resources
Classical genetics
null
overexpression
Large-scale survey
null
overexpression
Resources
69 total interaction(s) for 42 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 3
  • Affinity Capture-Western: 5
  • Biochemical Activity: 2
  • Co-fractionation: 2
  • Co-localization: 1
  • PCA: 29
  • Reconstituted Complex: 1
  • Two-hybrid: 7

Genetic Interactions
  • Dosage Growth Defect: 1
  • Negative Genetic: 9
  • Phenotypic Enhancement: 2
  • Phenotypic Suppression: 1
  • Positive Genetic: 3
  • Synthetic Lethality: 2
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 236
Molecular Weight (Da) 26,875
Isoelectric Point (pI) 10.65
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXV:48643 to 47933 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..711 48643..47933 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005507
SUMMARY PARAGRAPH for PEX11

Pex11p is a peroxin of the inner surface of the peroxisomal membrane and is required for peroxisome biogenesis (9, 6). Peroxisomes perform many essential functions in eukaryotic cells, and divide by budding from preexisting peroxisomes, a process that is not yet understood at the molecular level (4).

PEX11 proteins are widely conserved among eukaryotes, in which they are unique in their ability to promote peroxisome division (10, 11). Peroxisomal proliferation can be induced in S. cerevisiae by oleate, as PEX11 expression is regulated by oleate (4). The PEX11 promoter contains an oleate responsive element (ORE) overlapping a UAS1-like element, and it has been suggested that these elements in conjunction with their binding factors Pip2p-Oaf1p and Adr1p coordinate the carbon flux through beta-oxidation with peroxisome proliferation. Up-regulation of PEX11 is abolished in cells lacking Adr1p (6).

Pex11p in S. cerevisiae plays a primary role in medium-chain fatty acid beta-oxidation, a process that affects peroxisome number and size. Pex11p-deficient cells fail to increase peroxisome number in response to growth on fatty acids and instead accumulate a few giant peroxisomes. Mutants deficient in genes required for medium-chain fatty acid (MCFA) beta-oxidation display the same phenotype as Pex11p-deficient cells. The sole S. cerevisiae MCFA-CoA synthetase (Faa2p) remains properly localized to the inner leaflet of the peroxisomal membrane in PEX11 mutant cells. Therefore, the latency of MCFA activation observed in Pex11p-deficient cells suggests that Pex11p provides Faa2p with substrate. When PEX11 mutant cells are shifted from glucose to oleate-containing medium, an immediate deficiency in beta-oxidation of MCFAs occurs whereas giant peroxisomes and a failure to increase peroxisome abundance only became apparent much later (5).

Cells with a disrupted PEX11 gene grow well on glucose or ethanol, but fail to grow on oleate although peroxisomes were still induced by transfer to oleate-containing medium. The induced peroxisomes of pex11delta cells are fewer but considerably larger than wild-type. pex11delta cells cultured in oleate-containing medium form multiple buds, most of which are peroxisome-deficient. The growth defect of pex11delta cells on oleic acid appears to result from the inability to segregate the giant peroxisomes to daughter cells (3).

Pex25p and Pex27p have C-termini which are similar to the entire Pex11p, and localize to the peroxisomal membrane. Deletion of PEX25 or PEX27 also results in enlarged peroxisomes. A partial growth defect on fatty acids of a pex25delta mutant is not exacerbated by the additional deletion of PEX27; however, when PEX11 is also deleted, growth is abolished on all fatty acids. Moreover, the triple mutant strain exhibits a severe peroxisomal protein import defect (12). Overexpression of PEX27 or PEX25 leads to peroxisome proliferation and the formation of small peroxisomes. Overexpression of PEX11 has been reported to produce this same small-peroxisome phenotype (13), and also a similar phenotype in which cells contain an increased number of normal-sized peroxisomes (4).

Human have three PEX11 homologs - PEX11alpha, -beta, and -gamma - and PEX11 deficiencies in humans have been implicated in Zellweger syndrome, a lethal neurological disorder characterized by severe defects in peroxisomal protein import. The resulting defects in peroxisome metabolism and the accumulation of peroxisomal substrates are thought to cause the other Zellweger syndrome phenotypes, including neuronal migration defects, hypotonia, developmental delay, and neonatal lethality (14, 15).

Last updated: 2005-03-03 Contact SGD

References cited on this page View Complete Literature Guide for PEX11
1) Distel B, et al.  (1996) A unified nomenclature for peroxisome biogenesis factors. J Cell Biol 135(1):1-3
2) McCammon MT, et al.  (1990) Association of glyoxylate and beta-oxidation enzymes with peroxisomes of Saccharomyces cerevisiae. J Bacteriol 172(10):5816-27
3) Erdmann R and Blobel G  (1995) Giant peroxisomes in oleic acid-induced Saccharomyces cerevisiae lacking the peroxisomal membrane protein Pmp27p. J Cell Biol 128(4):509-23
4) Marshall PA, et al.  (1995) Pmp27 promotes peroxisomal proliferation. J Cell Biol 129(2):345-55
5) van Roermund CW, et al.  (2000) Pex11p plays a primary role in medium-chain fatty acid oxidation, a process that affects peroxisome number and size in Saccharomyces cerevisiae. J Cell Biol 150(3):489-98
6) Gurvitz A, et al.  (2001) Saccharomyces cerevisiae Adr1p governs fatty acid beta-oxidation and peroxisome proliferation by regulating POX1 and PEX11. J Biol Chem 276(34):31825-30
7) Knoblach B and Rachubinski RA  (2010) Phosphorylation-dependent Activation of Peroxisome Proliferator Protein PEX11 Controls Peroxisome Abundance. J Biol Chem 285(9):6670-80
8) Opalinski L, et al.  (2011) Membrane curvature during peroxisome fission requires Pex11. EMBO J 30(1):5-16
9) Marshall PA, et al.  (1996) Redox-sensitive homodimerization of Pex11p: a proposed mechanism to regulate peroxisomal division. J Cell Biol 135(1):123-37
10) Li X, et al.  (2002) PEX11alpha is required for peroxisome proliferation in response to 4-phenylbutyrate but is dispensable for peroxisome proliferator-activated receptor alpha-mediated peroxisome proliferation. Mol Cell Biol 22(23):8226-40
11) Li X and Gould SJ  (2002) PEX11 promotes peroxisome division independently of peroxisome metabolism. J Cell Biol 156(4):643-51
12) Rottensteiner H, et al.  (2003) Conserved function of pex11p and the novel pex25p and pex27p in peroxisome biogenesis. Mol Biol Cell 14(10):4316-28
13) Tam YY, et al.  (2003) Pex11-related proteins in peroxisome dynamics: a role for the novel peroxin Pex27p in controlling peroxisome size and number in Saccharomyces cerevisiae. Mol Biol Cell 14(10):4089-102
14) Li X, et al.  (2002) PEX11 beta deficiency is lethal and impairs neuronal migration but does not abrogate peroxisome function. Mol Cell Biol 22(12):4358-65
15) Tanaka A, et al.  (2003) cDNA cloning and characterization of the third isoform of human peroxin Pex11p. Biochem Biophys Res Commun 300(4):819-23