PET54/YGR222W Summary Help

Standard Name PET54 1
Systematic Name YGR222W
Feature Type ORF, Verified
Description Mitochondrial inner membrane protein; binds to the 5' UTR of the COX3 mRNA to activate its translation together with Pet122p and Pet494p; also binds to the COX1 Group I intron AI5 beta to facilitate exon ligation during splicing (1, 2, 3, 4)
Name Description PETite colonies
Chromosomal Location
ChrVII:939923 to 940804 | ORF Map | GBrowse
Gbrowse
Genetic position: 174 cM
Gene Ontology Annotations All PET54 GO evidence and references
  View Computational GO annotations for PET54
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 15 genes
Resources
Classical genetics
null
Large-scale survey
null
overexpression
Resources
26 total interaction(s) for 22 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 3
  • Affinity Capture-RNA: 3
  • Protein-RNA: 2
  • Two-hybrid: 4

Genetic Interactions
  • Dosage Rescue: 3
  • Negative Genetic: 5
  • Phenotypic Suppression: 1
  • Positive Genetic: 3
  • Synthetic Rescue: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 293
Molecular Weight (Da) 34,637
Isoelectric Point (pI) 10.01
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVII:939923 to 940804 | ORF Map | GBrowse
SGD ORF map
Genetic position: 174 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..882 939923..940804 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003454
References cited on this page View Complete Literature Guide for PET54
1) Costanzo MC, et al.  (1989) The PET54 gene of Saccharomyces cerevisiae: characterization of a nuclear gene encoding a mitochondrial translational activator and subcellular localization of its product. Genetics 122(2):297-305
2) Valencik ML, et al.  (1989) Disruption of the yeast nuclear PET54 gene blocks excision of mitochondrial intron aI5 beta from pre-mRNA for cytochrome c oxidase subunit I. EMBO J 8(12):3899-904
3) Naithani S, et al.  (2003) Interactions among COX1, COX2, and COX3 mRNA-specific translational activator proteins on the inner surface of the mitochondrial inner membrane of Saccharomyces cerevisiae. Mol Biol Cell 14(1):324-33
4) Kaspar BJ, et al.  (2008) A shared RNA-binding site in the Pet54 protein is required for translational activation and group I intron splicing in yeast mitochondria. Nucleic Acids Res 36(9):2958-68