PET309/YLR067C Summary Help

Standard Name PET309
Systematic Name YLR067C
Feature Type ORF, Verified
Description Specific translational activator for the COX1 mRNA; also influences stability of intron-containing COX1 primary transcripts; localizes to the mitochondrial inner membrane; contains seven pentatricopeptide repeats (PPRs) (1, 2 and see Summary Paragraph)
Name Description PETite colonies
Chromosomal Location
ChrXII:270710 to 267813 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All PET309 GO evidence and references
  View Computational GO annotations for PET309
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 4 genes
Resources
Classical genetics
null
reduction of function
Large-scale survey
null
Resources
54 total interaction(s) for 52 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 6
  • PCA: 1
  • Two-hybrid: 9

Genetic Interactions
  • Negative Genetic: 25
  • Phenotypic Suppression: 2
  • Positive Genetic: 3
  • Synthetic Growth Defect: 6
  • Synthetic Lethality: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 965
Molecular Weight (Da) 112,645
Isoelectric Point (pI) 10.11
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXII:270710 to 267813 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2898 270710..267813 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004057
SUMMARY PARAGRAPH for PET309

PET309 encodes an integral protein of the mitochondrial inner membrane (3) that is required for two steps in the expression of COX1, the mitochondrial gene encoding subunit 1 of cytochrome c oxidase. In strains where the COX1 gene contains introns, transcription or stability of intron-containing COX1 RNA species requires Pet309p (1). Translation of the COX1 mRNA also specifically requires Pet309p (1). The pet309 null mutant displays a respiratory growth defect, and Cox1p is missing from its mitochondrial translation products (1). An apparent ortholog exists in Neurospora crassa, where the CYA-5 protein, which has similarity to Pet309p, is also required for COX1 translation (4).

Last updated: 2002-08-01 Contact SGD

References cited on this page View Complete Literature Guide for PET309
1) Manthey GM and McEwen JE  (1995) The product of the nuclear gene PET309 is required for translation of mature mRNA and stability or production of intron-containing RNAs derived from the mitochondrial COX1 locus of Saccharomyces cerevisiae. EMBO J 14(16):4031-43
2) Tavares-Carreon F, et al.  (2008) The pentatricopeptide repeats present in Pet309 are necessary for translation but not for stability of the mitochondrial COX1 mRNA in yeast. J Biol Chem 283(3):1472-9
3) Manthey GM, et al.  (1998) The Saccharomyces cerevisiae Pet309 protein is embedded in the mitochondrial inner membrane. Eur J Biochem 255(1):156-61
4) Coffin JW, et al.  (1997) The Neurospora crassa cya-5 nuclear gene encodes a protein with a region of homology to the Saccharomyces cerevisiae PET309 protein and is required in a post-transcriptional step for the expression of the mitochondrially encoded COXI protein. Curr Genet 32(4):273-80