PEP8/YJL053W Summary Help

Standard Name PEP8
Systematic Name YJL053W
Alias GRD6 , VPS26 1 , 2 , VPT4 3
Feature Type ORF, Verified
Description Vacuolar protein component of the retromer; forms part of the multimeric membrane-associated retromer complex involved in vacuolar protein sorting along with Vps35p, Vps29p, Vps17p, and Vps5p; essential for endosome-to-Golgi retrograde protein transport; interacts with Ypt7p; protein abundance increases in response to DNA replication stress (4, 5, 6, 7)
Name Description carboxyPEPtidase Y-deficient 8
Chromosomal Location
ChrX:335898 to 337037 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All PEP8 GO evidence and references
  View Computational GO annotations for PEP8
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Mutant phenotypes All PEP8 Phenotype evidence and references
Classical genetics
null
reduction of function
Large-scale survey
null
Resources
interactions All PEP8 Interaction evidence and references
141 total interaction(s) for 91 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 12
  • Affinity Capture-Western: 16
  • Co-fractionation: 1
  • Co-purification: 3
  • PCA: 5
  • Reconstituted Complex: 1
Genetic Interactions
  • Dosage Lethality: 1
  • Negative Genetic: 58
  • Phenotypic Enhancement: 13
  • Phenotypic Suppression: 9
  • Positive Genetic: 11
  • Synthetic Growth Defect: 8
  • Synthetic Lethality: 3
Resources
Expression Summary
histogram
Resources
Protein Information All PEP8 Protein evidence and references
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrX:335898 to 337037 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..1140 335898..337037 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003589
References cited on this page View Complete Literature Guide for PEP8
1) Robinson JS, et al.  (1988) Protein sorting in Saccharomyces cerevisiae: isolation of mutants defective in the delivery and processing of multiple vacuolar hydrolases. Mol Cell Biol 8(11):4936-48
2) Rothman JH, et al.  (1989) Characterization of genes required for protein sorting and vacuolar function in the yeast Saccharomyces cerevisiae. EMBO J 8(7):2057-65
3) Bankaitis VA, et al.  (1986) Isolation of yeast mutants defective in protein targeting to the vacuole. Proc Natl Acad Sci U S A 83(23):9075-9
4) Seaman MN, et al.  (1998) A membrane coat complex essential for endosome-to-Golgi retrograde transport in yeast. J Cell Biol 142(3):665-81
5) Edgar AJ and Polak JM  (2000) Human homologues of yeast vacuolar protein sorting 29 and 35. Biochem Biophys Res Commun 277(3):622-30
6) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
7) Liu TT, et al.  (2012) Rab GTPase regulation of retromer-mediated cargo export during endosome maturation. Mol Biol Cell 23(13):2505-15
8) Jones EW  (1977) Proteinase mutants of Saccharomyces cerevisiae. Genetics 85(1):23-33