PEP8/YJL053W Summary Help

Standard Name PEP8 1
Systematic Name YJL053W
Alias GRD6 , VPS26 2 , 3 , VPT4 4
Feature Type ORF, Verified
Description Vacuolar protein component of the retromer; forms part of the multimeric membrane-associated retromer complex involved in vacuolar protein sorting along with Vps35p, Vps29p, Vps17p, and Vps5p; essential for endosome-to-Golgi retrograde protein transport; interacts with Ypt7p; protein abundance increases in response to DNA replication stress (5, 6, 7, 8)
Name Description carboxyPEPtidase Y-deficient 9
Chromosomal Location
ChrX:335898 to 337037 | ORF Map | GBrowse
Gene Ontology Annotations All PEP8 GO evidence and references
  View Computational GO annotations for PEP8
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 1 genes
Classical genetics
reduction of function
Large-scale survey
144 total interaction(s) for 94 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 13
  • Affinity Capture-Western: 16
  • Co-fractionation: 1
  • Co-purification: 3
  • PCA: 5
  • Reconstituted Complex: 1

Genetic Interactions
  • Dosage Lethality: 1
  • Negative Genetic: 59
  • Phenotypic Enhancement: 14
  • Phenotypic Suppression: 9
  • Positive Genetic: 11
  • Synthetic Growth Defect: 8
  • Synthetic Lethality: 3

Expression Summary
Length (a.a.) 379
Molecular Weight (Da) 42,547
Isoelectric Point (pI) 8.48
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrX:335898 to 337037 | ORF Map | GBrowse
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1140 335898..337037 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003589
References cited on this page View Complete Literature Guide for PEP8
1) Bachhawat AK, et al.  (1994) The yeast homolog of H < beta > 58, a mouse gene essential for embryogenesis, performs a role in the delivery of proteins to the vacuole. Genes Dev 8(12):1379-87
2) Robinson JS, et al.  (1988) Protein sorting in Saccharomyces cerevisiae: isolation of mutants defective in the delivery and processing of multiple vacuolar hydrolases. Mol Cell Biol 8(11):4936-48
3) Rothman JH, et al.  (1989) Characterization of genes required for protein sorting and vacuolar function in the yeast Saccharomyces cerevisiae. EMBO J 8(7):2057-65
4) Bankaitis VA, et al.  (1986) Isolation of yeast mutants defective in protein targeting to the vacuole. Proc Natl Acad Sci U S A 83(23):9075-9
5) Seaman MN, et al.  (1998) A membrane coat complex essential for endosome-to-Golgi retrograde transport in yeast. J Cell Biol 142(3):665-81
6) Edgar AJ and Polak JM  (2000) Human homologues of yeast vacuolar protein sorting 29 and 35. Biochem Biophys Res Commun 277(3):622-30
7) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
8) Liu TT, et al.  (2012) Rab GTPase regulation of retromer-mediated cargo export during endosome maturation. Mol Biol Cell 23(13):2505-15
9) Jones EW  (1977) Proteinase mutants of Saccharomyces cerevisiae. Genetics 85(1):23-33