PEP12/YOR036W Summary Help

Standard Name PEP12 1
Systematic Name YOR036W
Alias VPL6 2 , VPS6 3 , 4 , VPT13 3
Feature Type ORF, Verified
Description Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin (5, 6, 7, 8)
Name Description carboxyPEPtidase Y-deficient 9
Chromosomal Location
ChrXV:400348 to 401214 | ORF Map | GBrowse
Gbrowse
Genetic position: -5 cM
Gene Ontology Annotations All PEP12 GO evidence and references
  View Computational GO annotations for PEP12
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Mutant phenotypes All PEP12 Phenotype evidence and references
Classical genetics
null
reduction of function
Large-scale survey
null
overexpression
Resources
interactions All PEP12 Interaction evidence and references
125 total interaction(s) for 90 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 7
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 14
  • Co-fractionation: 2
  • Co-localization: 1
  • Co-purification: 4
  • PCA: 2
  • Reconstituted Complex: 6
  • Two-hybrid: 11
Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Lethality: 1
  • Dosage Rescue: 8
  • Negative Genetic: 29
  • Phenotypic Enhancement: 15
  • Positive Genetic: 2
  • Synthetic Growth Defect: 11
  • Synthetic Lethality: 8
  • Synthetic Rescue: 2
Resources
Expression Summary
histogram
Resources
Protein Information All PEP12 Protein evidence and references
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXV:400348 to 401214 | ORF Map | GBrowse
SGD ORF map
Genetic position: -5 cM
Last Update Coordinates: 2006-01-05 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..867 400348..401214 2006-01-05 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005562
References cited on this page View Complete Literature Guide for PEP12
1) Jones, E.  (1985) Personal Communication, Mortimer Map Edition 9
2) Rothman JH and Stevens TH  (1986) Protein sorting in yeast: mutants defective in vacuole biogenesis mislocalize vacuolar proteins into the late secretory pathway. Cell 47(6):1041-51
3) Robinson JS, et al.  (1988) Protein sorting in Saccharomyces cerevisiae: isolation of mutants defective in the delivery and processing of multiple vacuolar hydrolases. Mol Cell Biol 8(11):4936-48
4) Rothman JH, et al.  (1989) Characterization of genes required for protein sorting and vacuolar function in the yeast Saccharomyces cerevisiae. EMBO J 8(7):2057-65
5) Bassham DC, et al.  (1995) An Arabidopsis syntaxin homologue isolated by functional complementation of a yeast pep12 mutant. Proc Natl Acad Sci U S A 92(16):7262-6
6) Becherer KA, et al.  (1996) Novel syntaxin homologue, Pep12p, required for the sorting of lumenal hydrolases to the lysosome-like vacuole in yeast. Mol Biol Cell 7(4):579-94
7) Gotte M and Gallwitz D  (1997) High expression of the yeast syntaxin-related Vam3 protein suppresses the protein transport defects of a pep12 null mutant. FEBS Lett 411(1):48-52
8) Gerrard SR, et al.  (2000) Pep12p is a multifunctional yeast syntaxin that controls entry of biosynthetic, endocytic and retrograde traffic into the prevacuolar compartment. Traffic 1(3):259-69
9) Jones EW  (1977) Proteinase mutants of Saccharomyces cerevisiae. Genetics 85(1):23-33