PEP1/YBL017C Summary Help

Standard Name PEP1 1
Systematic Name YBL017C
Alias VPS10 2 , 3 , VPT1 4
Feature Type ORF, Verified
Description Type I transmembrane sorting receptor for multiple vacuolar hydrolases; cycles between the late-Golgi and prevacuolar endosome-like compartments (1, 5, 6 and see Summary Paragraph)
Name Description carboxyPEPtidase Y-deficient 7
Chromosomal Location
ChrII:191583 to 186844 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Genetic position: -8 cM
Gene Ontology Annotations All PEP1 GO evidence and references
  View Computational GO annotations for PEP1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 4 genes
Classical genetics
reduction of function
Large-scale survey
146 total interaction(s) for 113 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 14
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 3
  • Co-fractionation: 6
  • Co-purification: 1
  • PCA: 15
  • Two-hybrid: 2

Genetic Interactions
  • Dosage Rescue: 3
  • Negative Genetic: 89
  • Phenotypic Enhancement: 3
  • Phenotypic Suppression: 1
  • Positive Genetic: 4
  • Synthetic Rescue: 2

Expression Summary
Length (a.a.) 1,579
Molecular Weight (Da) 177,776
Isoelectric Point (pI) 4.59
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrII:191583 to 186844 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Genetic position: -8 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1997-01-28
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..4740 191583..186844 2011-02-03 1997-01-28
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000113

PEP1 (also known as VPS10 and VPT1) encodes a sorting receptor that functions in targeting mutiple vacuolar proteases, such as carboxypeptidase Y (Prc1p), proteinase A (Pep4p), and aminopeptidase Y (Ape3p), to the vacuole (1, 8, 9). Pep1p is a type I transmembrane protein that cycles between the late Golgi and the endosome (1, 10). Pep1p and associated ligands are sorted from the Golgi to the endosome via clathrin-coated vesicles (11), while recycling Pep1p back to the late Golgi is mediated by the retromer complex (comprised of Vps35p, Vps29p, Vps5p, Vps17p, and Pep8p) (12, 13, 14). Proper Pep1p localization is also dependent on Vps8p and Vps13p (15, 16). pep1 null mutants are defective in sorting and processing of vacuolar proteins, with vacuolar proteases being missorted to the cell surface (4, 17, 3, 2, 6). Overexpression of VTH2, which encodes a protein homologous to Pep1p, complements the pep1-delta missorting defects (6). Other proteins with similarity to Pep1p have been identified in fission yeast, rabbit, chicken, and man (18, 19, 20).

Last updated: 2011-02-07 Contact SGD

References cited on this page View Complete Literature Guide for PEP1
1) Marcusson EG, et al.  (1994) The sorting receptor for yeast vacuolar carboxypeptidase Y is encoded by the VPS10 gene. Cell 77(4):579-86
2) Robinson JS, et al.  (1988) Protein sorting in Saccharomyces cerevisiae: isolation of mutants defective in the delivery and processing of multiple vacuolar hydrolases. Mol Cell Biol 8(11):4936-48
3) Rothman JH, et al.  (1989) Characterization of genes required for protein sorting and vacuolar function in the yeast Saccharomyces cerevisiae. EMBO J 8(7):2057-65
4) Bankaitis VA, et al.  (1986) Isolation of yeast mutants defective in protein targeting to the vacuole. Proc Natl Acad Sci U S A 83(23):9075-9
5) Cereghino JL, et al.  (1995) The cytoplasmic tail domain of the vacuolar protein sorting receptor Vps10p and a subset of VPS gene products regulate receptor stability, function, and localization. Mol Biol Cell 6(9):1089-102
6) Cooper AA and Stevens TH  (1996) Vps10p cycles between the late-Golgi and prevacuolar compartments in its function as the sorting receptor for multiple yeast vacuolar hydrolases. J Cell Biol 133(3):529-41
7) Jones EW  (1977) Proteinase mutants of Saccharomyces cerevisiae. Genetics 85(1):23-33
8) Westphal V, et al.  (1996) Multiple pathways for vacuolar sorting of yeast proteinase A. J Biol Chem 271(20):11865-70
9) Jorgensen MU, et al.  (1999) Ligand recognition and domain structure of Vps10p, a vacuolar protein sorting receptor in Saccharomyces cerevisiae. Eur J Biochem 260(2):461-9
10) Nothwehr SF and Hindes AE  (1997) The yeast VPS5/GRD2 gene encodes a sorting nexin-1-like protein required for localizing membrane proteins to the late Golgi. J Cell Sci 110 ( Pt 9)():1063-72
11) Deloche O, et al.  (2001) Vps10p transport from the trans-Golgi network to the endosome is mediated by clathrin-coated vesicles. Mol Biol Cell 12(2):475-85
12) Seaman MN, et al.  (1998) A membrane coat complex essential for endosome-to-Golgi retrograde transport in yeast. J Cell Biol 142(3):665-81
13) Reddy JV and Seaman MN  (2001) Vps26p, a component of retromer, directs the interactions of Vps35p in endosome-to-Golgi retrieval. Mol Biol Cell 12(10):3242-56
14) Nothwehr SF, et al.  (1999) Distinct domains within Vps35p mediate the retrieval of two different cargo proteins from the yeast prevacuolar/endosomal compartment. Mol Biol Cell 10(4):875-90
15) Chen YJ and Stevens TH  (1996) The VPS8 gene is required for localization and trafficking of the CPY sorting receptor in Saccharomyces cerevisiae. Eur J Cell Biol 70(4):289-97
16) Brickner JH and Fuller RS  (1997) SOI1 encodes a novel, conserved protein that promotes TGN-endosomal cycling of Kex2p and other membrane proteins by modulating the function of two TGN localization signals. J Cell Biol 139(1):23-36
17) Banta LM, et al.  (1988) Organelle assembly in yeast: characterization of yeast mutants defective in vacuolar biogenesis and protein sorting. J Cell Biol 107(4):1369-83
18) Iwaki T, et al.  (2006) Vacuolar protein sorting receptor in Schizosaccharomyces pombe. Microbiology 152(Pt 5):1523-32
19) Yamazaki H, et al.  (1996) Elements of neural adhesion molecules and a yeast vacuolar protein sorting receptor are present in a novel mammalian low density lipoprotein receptor family member. J Biol Chem 271(40):24761-8
20) Yamazaki H, et al.  (1997) A novel member of the LDL receptor gene family with eleven binding repeats is structurally related to neural adhesion molecules and a yeast vacuolar protein sorting receptor. J Atheroscler Thromb 4(1):20-6