PDR12/YPL058C Summary Help

Standard Name PDR12
Systematic Name YPL058C
Feature Type ORF, Verified
Description Plasma membrane ATP-binding cassette (ABC) transporter; weak-acid-inducible multidrug transporter required for weak organic acid resistance; induced by sorbate and benzoate and regulated by War1p; mutants exhibit sorbate hypersensitivity (1, 2, 3, 4 and see Summary Paragraph)
Name Description Pleiotropic Drug Resistance
Chromosomal Location
ChrXVI:450377 to 445842 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All PDR12 GO evidence and references
  View Computational GO annotations for PDR12
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 5 genes
Classical genetics
Large-scale survey
101 total interaction(s) for 80 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 9
  • Affinity Capture-RNA: 1
  • PCA: 62
  • Two-hybrid: 1

Genetic Interactions
  • Dosage Growth Defect: 3
  • Dosage Rescue: 1
  • Negative Genetic: 18
  • Phenotypic Suppression: 1
  • Synthetic Growth Defect: 3
  • Synthetic Rescue: 2

Expression Summary
Length (a.a.) 1,511
Molecular Weight (Da) 171,064
Isoelectric Point (pI) 6.82
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXVI:450377 to 445842 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..4536 450377..445842 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | TCDB | UniProtKB
Primary SGDIDS000005979

PDR12 encodes an ATP-binding cassette (ABC) transporter which is required for the development of weak organic acid resistance by mediating the energy-dependent extrusion of water-soluble carboxylate anions from the cell (5). Located in the plasma membrane, Pdr12p appears to confer resistance to those carboxylic acids that partition into both the lipid bilayer and aqueous phases (6), especially water-soluble, monocarboxylic acids with chain lengths of from C(1) to C(7) (2).

PDR12 is strongly induced by sorbate, benzoate and certain other moderately lipophilic carboxylate compounds, but not by organic alcohols or high levels of acetate (6). The PDR12 promoter contains a cis-acting weak acid response element (WARE) which is required for its induction (7). This WARE is the major target of transcription factor War1p under sorbic acid stress (4). War1p occupies the site in the both presence and absence of stress, demonstrating constitutive DNA binding, and suggesting that weak acid stress, such as that produced by sorbate or benzoate, triggers phosphorylation and possible activation of War1p (7). In turn, War1p activation allows the induction of PDR12 through a novel signal transduction event that elicits weak organic acid stress adaptation through the active efflux of weak organic acids from the cytosol (7), (1).

Last updated: 2004-07-22 Contact SGD

References cited on this page View Complete Literature Guide for PDR12
1) Holyoak CD, et al.  (2000) Loss of Cmk1 Ca(2+)-calmodulin-dependent protein kinase in yeast results in constitutive weak organic acid resistance, associated with a post-transcriptional activation of the Pdr12 ATP-binding cassette transporter. Mol Microbiol 37(3):595-605
2) Holyoak CD, et al.  (1999) The Saccharomyces cerevisiae weak-acid-inducible ABC transporter Pdr12 transports fluorescein and preservative anions from the cytosol by an energy-dependent mechanism. J Bacteriol 181(15):4644-52
3) Decottignies A and Goffeau A  (1997) Complete inventory of the yeast ABC proteins. Nat Genet 15(2):137-45
4) Schuller C, et al.  (2004) Global phenotypic analysis and transcriptional profiling defines the weak acid stress response regulon in Saccharomyces cerevisiae. Mol Biol Cell 15(2):706-20
5) Piper P, et al.  (1998) The pdr12 ABC transporter is required for the development of weak organic acid resistance in yeast. EMBO J 17(15):4257-65
6) Hatzixanthis K, et al.  (2003) Moderately lipophilic carboxylate compounds are the selective inducers of the Saccharomyces cerevisiae Pdr12p ATP-binding cassette transporter. Yeast 20(7):575-85
7) Kren A, et al.  (2003) War1p, a novel transcription factor controlling weak acid stress response in yeast. Mol Cell Biol 23(5):1775-85