PDI1/YCL043C Summary Help

Standard Name PDI1 1
Systematic Name YCL043C
Alias MFP1 , TRG1
Feature Type ORF, Verified
Description Protein disulfide isomerase; multifunctional protein of ER lumen, essential for formation of disulfide bonds in secretory and cell-surface proteins, unscrambles non-native disulfide bonds; key regulator of Ero1p; forms complex with Mnl1p that has exomannosidase activity, processing unfolded protein-bound Man8GlcNAc2 oligosaccharides to Man7GlcNAc2, promoting degradation in unfolded protein response; PDI1 has a paralog, EUG1, that arose from the whole genome duplication (1, 2, 3, 4, 5, 6, 7)
Name Description Protein Disulfide Isomerase 8
Chromosomal Location
ChrIII:50221 to 48653 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All PDI1 GO evidence and references
  View Computational GO annotations for PDI1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 7 genes
Classical genetics
reduction of function
Large-scale survey
reduction of function
200 total interaction(s) for 161 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 23
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 7
  • Biochemical Activity: 7
  • Co-crystal Structure: 1
  • PCA: 1
  • Protein-peptide: 2
  • Reconstituted Complex: 1

Genetic Interactions
  • Dosage Lethality: 1
  • Dosage Rescue: 7
  • Negative Genetic: 19
  • Phenotypic Suppression: 2
  • Positive Genetic: 5
  • Synthetic Lethality: 119
  • Synthetic Rescue: 4

Expression Summary
Length (a.a.) 522
Molecular Weight (Da) 58,227
Isoelectric Point (pI) 4.22
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrIII:50221 to 48653 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2000-09-13 | Sequence: 1997-01-28
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1569 50221..48653 2000-09-13 1997-01-28
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000548
References cited on this page View Complete Literature Guide for PDI1
1) Farquhar R, et al.  (1991) Protein disulfide isomerase is essential for viability in Saccharomyces cerevisiae. Gene 108(1):81-9
2) Laboissiere MC, et al.  (1995) The essential function of protein-disulfide isomerase is to unscramble non-native disulfide bonds. J Biol Chem 270(47):28006-9
3) Sevier CS, et al.  (2001) A flavoprotein oxidase defines a new endoplasmic reticulum pathway for biosynthetic disulphide bond formation. Nat Cell Biol 3(10):874-82
4) Noiva R and Lennarz WJ  (1992) Protein disulfide isomerase. A multifunctional protein resident in the lumen of the endoplasmic reticulum. J Biol Chem 267(6):3553-6
5) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
6) Gauss R, et al.  (2011) A complex of pdi1p and the mannosidase htm1p initiates clearance of unfolded glycoproteins from the endoplasmic reticulum. Mol Cell 42(6):782-93
7) Kim S, et al.  (2012) Balanced Ero1 activation and inactivation establishes ER redox homeostasis. J Cell Biol 196(6):713-25
8) Mizunaga T, et al.  (1990) Purification and characterization of yeast protein disulfide isomerase. J Biochem 108(5):846-51