PCL8/YPL219W Summary Help

Standard Name PCL8 1
Systematic Name YPL219W
Feature Type ORF, Verified
Description Cyclin; interacts with Pho85p cyclin-dependent kinase (Cdk) to phosphorylate and regulate glycogen synthase, also activates Pho85p for Glc8p phosphorylation; PCL8 has a paralog, PCL10, that arose from the whole genome duplication (1, 2, 3, 4, 5)
Name Description Pho85 CycLin 1
Chromosomal Location
ChrXVI:136750 to 138228 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All PCL8 GO evidence and references
  View Computational GO annotations for PCL8
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 3 genes
Resources
Classical genetics
null
Large-scale survey
null
Resources
45 total interaction(s) for 35 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 10
  • Reconstituted Complex: 1
  • Two-hybrid: 7

Genetic Interactions
  • Dosage Growth Defect: 4
  • Dosage Rescue: 5
  • Negative Genetic: 10
  • Phenotypic Suppression: 1
  • Positive Genetic: 6
  • Synthetic Lethality: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 492
Molecular Weight (Da) 55,430
Isoelectric Point (pI) 4.51
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXVI:136750 to 138228 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1479 136750..138228 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000006140
References cited on this page View Complete Literature Guide for PCL8
1) Measday V, et al.  (1997) A family of cyclin-like proteins that interact with the Pho85 cyclin-dependent kinase. Mol Cell Biol 17(3):1212-23
2) Huang D, et al.  (1998) Cyclin partners determine Pho85 protein kinase substrate specificity in vitro and in vivo: control of glycogen biosynthesis by Pcl8 and Pcl10. Mol Cell Biol 18(6):3289-99
3) Wilson WA, et al.  (2002) Analysis of respiratory mutants reveals new aspects of the control of glycogen accumulation by the cyclin-dependent protein kinase Pho85p. FEBS Lett 515(1-3):104-8
4) Tan YS, et al.  (2003) Pho85 phosphorylates the Glc7 protein phosphatase regulator Glc8 in vivo. J Biol Chem 278(1):147-53
5) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61