PCL2/YDL127W Summary Help

Standard Name PCL2 1
Systematic Name YDL127W
Alias CLN4
Feature Type ORF, Verified
Description Cyclin, interacts with cyclin-dependent kinase Pho85p; member of the Pcl1,2-like subfamily, involved in the regulation of polarized growth and morphogenesis and progression through the cell cycle; localizes to sites of polarized cell growth; PCL2 has a paralog, PCL9, that arose from the whole genome duplication (1, 2, 3, 4, 5 and see Summary Paragraph)
Name Description Pho85 CycLin 1
Chromosomal Location
ChrIV:234927 to 235853 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All PCL2 GO evidence and references
  View Computational GO annotations for PCL2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 10 genes
Resources
Classical genetics
null
Large-scale survey
null
Resources
51 total interaction(s) for 35 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 1
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 2
  • Biochemical Activity: 1
  • Protein-RNA: 1
  • Reconstituted Complex: 3
  • Two-hybrid: 10

Genetic Interactions
  • Dosage Growth Defect: 2
  • Dosage Lethality: 3
  • Dosage Rescue: 4
  • Negative Genetic: 8
  • Phenotypic Enhancement: 7
  • Synthetic Growth Defect: 2
  • Synthetic Lethality: 5

Resources
Expression Summary
histogram
Resources
Length (a.a.) 308
Molecular Weight (Da) 35,207
Isoelectric Point (pI) 10.52
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:234927 to 235853 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2004-02-11 | Sequence: 2004-02-11
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..927 234927..235853 2004-02-11 2004-02-11
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002285
SUMMARY PARAGRAPH for PCL2

Pcl1p and Pcl2p are cyclins that interact with the cyclin-dependent kinase (CDK) Pho85p (5, 6). They belong to the Pcl1,2 subfamily of cyclins, which also includes Pcl5p, Pcl9p, and Clg1p (2). Except for Pcl5p, members of the Pcl1,2 subfamily are primarily required for progression through the cell cycle and regulating cell polarity and morphogenesis (2, 5, 6). Abnormal morphological phenotypes begin to appear in pcl1 pcl2 pcl9 triple mutants, suggesting that members of the Pcl1,2 subfamily share a common function (2). PCL1 and PCL2 are required for the phosphorylation of key regulators of polarized growth, septin ring assembly, and morphogenesis in G1 (7, 8, 9, 10). Some targets, such as Rga2p and Bni4p, have been shown to be phosphorylated by both Pcl1p-Pho85p and Pcl2p-Pho85p in vitro (8, 10). Mutations in genes that encode these substrates in combination with a pcl1 pcl2 double mutant result in severe growth defects (8, 9, 10).

Pcl1p and Pcl2p have some redundancy with Cln1p and Cln2p, two G1-cyclins that interact with CDK Cdc28p (5, 6). Pcl1p and Pcl2p are required for cell cycle progression in the absence of Cln1p and Cln2p; a strain lacking any 3 of the 4 cyclins is still viable (11, 1, 3). In further support of PCL1 and PCL2 encoding a parallel pathway to CLN1 and CLN2, many substrates of Pcl1p-Pho85p and Pcl2p-Pho85p are also substrates of Cln1p-Cdc28p and/or Cln2p-Cdc28p or have growth defects when mutated in a strain containing a cln1 cln2 double mutant (8, 9, 10).

Corresponding to their role in G1, PCL1 and PCL2 expression and activity peak in G1 (11, 1, 2). Expression of PCL1, like CLN1 and CLN2, is regulated by Swi4p, Bck2p, and the Sin3p-Rpd3p histone deacetylase complex (12, 13, 14). Consistent with their role in regulating morphogenesis and polarized growth, Pcl1p and Pcl2p are localized to the bud neck and sites of polarized growth (3).

Last updated: 2009-07-08 Contact SGD

References cited on this page View Complete Literature Guide for PCL2
1) Measday V, et al.  (1994) The PCL2 (ORFD)-PHO85 cyclin-dependent kinase complex: a cell cycle regulator in yeast. Science 266(5189):1391-5
2) Measday V, et al.  (1997) A family of cyclin-like proteins that interact with the Pho85 cyclin-dependent kinase. Mol Cell Biol 17(3):1212-23
3) Moffat J and Andrews B  (2004) Late-G1 cyclin-CDK activity is essential for control of cell morphogenesis in budding yeast. Nat Cell Biol 6(1):59-66
4) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
5) Huang D, et al.  (2007) Pho85, a multifunctional cyclin-dependent protein kinase in budding yeast. Mol Microbiol 66(2):303-14
6) Carroll AS and O'Shea EK  (2002) Pho85 and signaling environmental conditions. Trends Biochem Sci 27(2):87-93
7) Friesen H, et al.  (2003) Regulation of the yeast amphiphysin homologue Rvs167p by phosphorylation. Mol Biol Cell 14(7):3027-40
8) Sopko R, et al.  (2007) Activation of the Cdc42p GTPase by cyclin-dependent protein kinases in budding yeast. EMBO J 26(21):4487-500
9) Egelhofer TA, et al.  (2008) The septins function in G1 pathways that influence the pattern of cell growth in budding yeast. PLoS One 3(4):e2022
10) Zou J, et al.  (2009) Regulation of cell polarity through phosphorylation of Bni4 by Pho85 G1 cyclin-dependent kinases in Saccharomyces cerevisiae. Mol Biol Cell 20(14):3239-50
11) Fernandez-Sarabia MJ, et al.  (1992) SIT4 protein phosphatase is required for the normal accumulation of SWI4, CLN1, CLN2, and HCS26 RNAs during late G1. Genes Dev 6(12A):2417-28
12) Ogas J, et al.  (1991) Transcriptional activation of CLN1, CLN2, and a putative new G1 cyclin (HCS26) by SWI4, a positive regulator of G1-specific transcription. Cell 66(5):1015-26
13) Di Como CJ, et al.  (1995) Activation of CLN1 and CLN2 G1 cyclin gene expression by BCK2. Mol Cell Biol 15(4):1835-46
14) Stephan O and Koch C  (2009) Sin3 is involved in cell size control at Start in Saccharomyces cerevisiae. FEBS J 276(14):3810-24