PAH1/YMR165C Summary 
| Standard Name | PAH1 1 |
|---|---|
| Systematic Name | YMR165C |
| Alias | SMP2 2 |
| Feature Type | ORF, Verified |
| Description | Mg2+-dependent phosphatidate (PA) phosphatase; dephosphorylates PA to yield diacylglycerol; responsible for de novo lipid synthesis and formation of lipid droplets; phosphorylation by Pho80p-Pho85p decreases catalytic activity and alters Pah1p localization and abundance; phosphorylation by protein kinase A decreases catalytic efficiency; dephosphorylation by Nem1p-Spo7p anchors Pah1p to the membrane increasing substrate catalysis; homologous to mammalian lipins 1 and 2 (1, 3, 4, 5, 6, 7, 8) |
| Name Description | Phosphatidic Acid phosphoHydrolase 1 |
| Chromosomal Location | |
|---|---|
| Note: this feature is encoded on the Crick strand. | |
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Gene Ontology Annotations All PAH1 GO evidence and references
| View Computational GO annotations for PAH1 | |
| Molecular Function | |
| Manually curated | |
| Biological Process | |
| Manually curated | |
| Cellular Component | |
| Manually curated | |
| High-throughput |
| Pathways |
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Mutant phenotypes All PAH1 Phenotype evidence and references
| Classical genetics | |
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| conditional |
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| null |
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| overexpression | |
| unspecified | |
| Large-scale survey | |
| null |
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| overexpression | |
| unspecified | |
| Resources |
interactions All PAH1 Interaction evidence and references
| 146 total interaction(s) for 127 unique genes/features. | |
| Physical Interactions |
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| Genetic Interactions |
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| Resources |
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Expression Summary
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| Resources |
Protein Information All PAH1 Protein evidence and references
| Localization | |
|---|---|
| Phosphorylation | PhosphoGRID | PhosphoPep Database |
| Structure | |
| Homologs |
sequence information
| Note: this feature is encoded on the Crick strand. | |||||||||||||
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| Last Update | Coordinates: 2011-02-03 | Sequence: 1996-07-31 | ||||||||||||
| Subfeature details |
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| Retrieve sequences | |||||||||||||
Analyze Sequence
| S288C only | |
|---|---|
| S288C vs. other species | |
| S288C vs. other strains |
Resources
| External Links | All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB |
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| Primary SGDID | S000004775 |
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References cited on this page View Complete Literature Guide for PAH1
| 1) | Han GS, et al. (2006) The Saccharomyces cerevisiae Lipin homolog is a Mg2+-dependent phosphatidate phosphatase enzyme. J Biol Chem 281(14):9210-8 |
| 2) | Irie K, et al. (1993) A gene, SMP2, involved in plasmid maintenance and respiration in Saccharomyces cerevisiae encodes a highly charged protein. Mol Gen Genet 236(2-3):283-8 |
| 3) | Santos-Rosa H, et al. (2005) The yeast lipin Smp2 couples phospholipid biosynthesis to nuclear membrane growth. EMBO J 24(11):1931-41 |
| 4) | Karanasios E, et al. (2010) A phosphorylation-regulated amphipathic helix controls the membrane translocation and function of the yeast phosphatidate phosphatase. Proc Natl Acad Sci U S A 107(41):17539-44 |
| 5) | Adeyo O, et al. (2011) The yeast lipin orthologue Pah1p is important for biogenesis of lipid droplets. J Cell Biol 192(6):1043-55 |
| 6) | Choi HS, et al. (2012) Pho85p-Pho80p phosphorylation of yeast Pah1p phosphatidate phosphatase regulates its activity, location, abundance, and function in lipid metabolism. J Biol Chem 287(14):11290-301 |
| 7) | Su WM, et al. (2012) Protein kinase A-mediated phosphorylation of Pah1p phosphatidate phosphatase functions in conjunction with the Pho85p-Pho80p and Cdc28p-cyclin B kinases to regulate lipid synthesis in yeast. J Biol Chem 287(40):33364-76 |
| 8) | Grimsey N, et al. (2008) Temporal and spatial regulation of the phosphatidate phosphatases lipin 1 and 2. J Biol Chem 283(43):29166-74 |
