PAH1/YMR165C Summary Help

Standard Name PAH1 1
Systematic Name YMR165C
Alias SMP2 2
Feature Type ORF, Verified
Description Mg2+-dependent phosphatidate (PA) phosphatase; dephosphorylates PA to yield diacylglycerol; responsible for de novo lipid synthesis and formation of lipid droplets; phosphorylation by Pho80p-Pho85p decreases catalytic activity and alters Pah1p localization and abundance; phosphorylation by protein kinase A decreases catalytic efficiency; dephosphorylation by Nem1p-Spo7p anchors Pah1p to the membrane increasing substrate catalysis; homologous to mammalian lipins 1 and 2 (1, 3, 4, 5, 6, 7, 8)
Name Description Phosphatidic Acid phosphoHydrolase 1
Chromosomal Location
ChrXIII:592628 to 590040 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All PAH1 GO evidence and references
  View Computational GO annotations for PAH1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 5 genes
Resources
Pathways
Classical genetics
conditional
null
overexpression
reduction of function
unspecified
Large-scale survey
null
overexpression
unspecified
Resources
151 total interaction(s) for 128 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 3
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 1
  • Biochemical Activity: 37
  • PCA: 1
  • Protein-RNA: 1
  • Reconstituted Complex: 1
  • Two-hybrid: 1

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Rescue: 4
  • Negative Genetic: 53
  • Phenotypic Enhancement: 6
  • Phenotypic Suppression: 5
  • Positive Genetic: 13
  • Synthetic Growth Defect: 11
  • Synthetic Lethality: 10
  • Synthetic Rescue: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 862
Molecular Weight (Da) 95,030
Isoelectric Point (pI) 4.68
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIII:592628 to 590040 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2589 592628..590040 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004775
References cited on this page View Complete Literature Guide for PAH1
1) Han GS, et al.  (2006) The Saccharomyces cerevisiae Lipin homolog is a Mg2+-dependent phosphatidate phosphatase enzyme. J Biol Chem 281(14):9210-8
2) Irie K, et al.  (1993) A gene, SMP2, involved in plasmid maintenance and respiration in Saccharomyces cerevisiae encodes a highly charged protein. Mol Gen Genet 236(2-3):283-8
3) Santos-Rosa H, et al.  (2005) The yeast lipin Smp2 couples phospholipid biosynthesis to nuclear membrane growth. EMBO J 24(11):1931-41
4) Karanasios E, et al.  (2010) A phosphorylation-regulated amphipathic helix controls the membrane translocation and function of the yeast phosphatidate phosphatase. Proc Natl Acad Sci U S A 107(41):17539-44
5) Adeyo O, et al.  (2011) The yeast lipin orthologue Pah1p is important for biogenesis of lipid droplets. J Cell Biol 192(6):1043-55
6) Choi HS, et al.  (2012) Pho85p-Pho80p phosphorylation of yeast Pah1p phosphatidate phosphatase regulates its activity, location, abundance, and function in lipid metabolism. J Biol Chem 287(14):11290-301
7) Su WM, et al.  (2012) Protein kinase A-mediated phosphorylation of Pah1p phosphatidate phosphatase functions in conjunction with the Pho85p-Pho80p and Cdc28p-cyclin B kinases to regulate lipid synthesis in yeast. J Biol Chem 287(40):33364-76
8) Grimsey N, et al.  (2008) Temporal and spatial regulation of the phosphatidate phosphatases lipin 1 and 2. J Biol Chem 283(43):29166-74