OLI1/Q0130 Summary Help

Standard Name OLI1 1
Systematic Name Q0130
Alias ATP9 , OLI3 2
Feature Type ORF, Verified
Description F0-ATP synthase subunit c (ATPase-associated proteolipid); encoded on the mitochondrial genome; mutation confers oligomycin resistance; expression is specifically dependent on the nuclear genes AEP1 and AEP2 (1, 3, 4, 5 and see Summary Paragraph)
Name Description OLIgomycin resistance 1
Chromosomal Location
ChrMito:46723 to 46953 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All OLI1 GO evidence and references
  View Computational GO annotations for OLI1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 1 genes
Resources
Classical genetics
conditional
reduction of function
Resources
16 total interaction(s) for 10 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 5
  • Affinity Capture-Western: 6
  • Co-crystal Structure: 1
  • Co-fractionation: 1
  • Co-purification: 2
  • Reconstituted Complex: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 76
Molecular Weight (Da) 7,759
Isoelectric Point (pI) 8.42
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrMito:46723 to 46953 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2000-05-19 | Sequence: 2000-05-19
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..231 46723..46953 2000-05-19 2000-05-19
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000007274
SUMMARY PARAGRAPH for OLI1

OLI1 is one of three mitochondrial genes, along with ATP8 and ATP6, that encode ATP synthase subunits. Also known as subunit 9 or Y9, Oli1p is homologous to subunit c of bacterial ATP synthase (6 and references therein). The ATP synthase complex utilizes proton motive force to generate ATP from ADP and Pi (7). The structure of this enzyme complex is highly conserved among diverse organisms and consists of two major components, soluble F1 and membrane-bound F0, each of which contains many subunits. The F0 proton pore is formed by a ring of twelve Y9 subunits. It is hypothesized that ATP synthase activity is driven by the rotation of the Y9 ring and subsequent transfer of that rotational force to the F1 catalytic core (7 and 8).

Although OLI1 is essential for ATP synthase function, it is not essential for life in yeast. Deletion of OLI1, like deletions in many genes necessary for the function or maintenance of mitochondria, leads to a "petite" phenotype that is slow-growing and unable to survive on nonfermentable carbon sources (9).

General ATP synthase structure and function are reviewed in references 7 and 8. For a review that is specific to yeast, see reference 6.

Last updated: 2006-04-03 Contact SGD

References cited on this page View Complete Literature Guide for OLI1
1) Trembath MK, et al.  (1975) Biogenesis of mitochondria 36, The genetic and biochemical analysis of a mitochondrially determined cold sensitive oligomycin resistant mutant of Saccharomyces cerevisiae with affected mitochondrial ATPase assembly. Mol Gen Genet 141(1):9-22
2) Ooi BG, et al.  (1985) DNA sequence analysis of the oli1 gene reveals amino acid changes in mitochondrial ATPase subunit 9 from oligomycin-resistant mutants of Saccharomyces cerevisiae. Eur J Biochem 152(3):709-14
3) Nagley P, et al.  (1986) Amino acid substitutions in mitochondrial ATPase subunit 9 of Saccharomyces cerevisiae leading to oligomycin or venturicidin resistance. FEBS Lett 195(1-2):159-63
4) Payne MJ, et al.  (1993) Characterization of a second nuclear gene, AEP1, required for expression of the mitochondrial OLI1 gene in Saccharomyces cerevisiae. Curr Genet 24(1-2):126-35
5) Finnegan PM, et al.  (1991) Characterization of a yeast nuclear gene, AEP2, required for accumulation of mitochondrial mRNA encoding subunit 9 of the ATP synthase. Curr Genet 20(1-2):53-61
6) Devenish RJ, et al.  (2000) Insights into ATP synthase assembly and function through the molecular genetic manipulation of subunits of the yeast mitochondrial enzyme complex. Biochim Biophys Acta 1458(2-3):428-42
7) Boyer PD  (1997) The ATP synthase--a splendid molecular machine. Annu Rev Biochem 66:717-49
8) Nakamoto RK, et al.  (1999) Rotational coupling in the F0F1 ATP synthase. Annu Rev Biophys Biomol Struct 28():205-34
9) Somlo M, et al.  (1977) An oligomycin-resistant adenosine triphosphatase and its effects on cellular growth, mitochondrial oxidative phosphorylation and respiratory proton translocation in Saccharomyces cerevisiae. Biochem J 162(1):51-9