NUT2/YPR168W Summary Help

Standard Name NUT2 1
Systematic Name YPR168W
Alias MED10
Feature Type ORF, Verified
Description Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for transcriptional activation and has a role in basal transcription; protein abundance increases in response to DNA replication stress (2, 3)
Name Description Negative regulation of URS Two 1
Chromosomal Location
ChrXVI:878076 to 878549 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All NUT2 GO evidence and references
  View Computational GO annotations for NUT2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 4 genes
Resources
Classical genetics
null
Large-scale survey
null
reduction of function
Resources
102 total interaction(s) for 43 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 58
  • Affinity Capture-Western: 16
  • Co-purification: 1
  • PCA: 3
  • Reconstituted Complex: 1
  • Two-hybrid: 13

Genetic Interactions
  • Negative Genetic: 1
  • Phenotypic Suppression: 2
  • Synthetic Lethality: 2
  • Synthetic Rescue: 5

Resources
Expression Summary
histogram
Resources
Length (a.a.) 157
Molecular Weight (Da) 17,908
Isoelectric Point (pI) 4.71
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXVI:878076 to 878549 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..474 878076..878549 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000006372
References cited on this page View Complete Literature Guide for NUT2
1) Tabtiang RK and Herskowitz I  (1998) Nuclear proteins Nut1p and Nut2p cooperate to negatively regulate a Swi4p-dependent lacZ reporter gene in Saccharomyces cerevisiae. Mol Cell Biol 18(8):4707-18
2) Gustafsson CM, et al.  (1998) Identification of new mediator subunits in the RNA polymerase II holoenzyme from Saccharomyces cerevisiae. J Biol Chem 273(47):30851-4
3) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76